Gene expression pattern in Caco-2 cells following rotavirus infection

Journal of Virology
Mariela A CuadrasH B Greenberg

Abstract

Rotaviruses are recognized as the leading cause of severe dehydrating diarrhea in infants and young children worldwide. Preventive and therapeutic strategies are urgently needed to fight this pathogen. In tissue culture and in vivo, rotavirus induces structural and functional alterations in the host cell. In order to better understand the molecular mechanisms involved in the events after rotavirus infection, we identified host cellular genes whose mRNA levels changed after infection. For this analysis, we used microarrays containing more than 38,000 human cDNAs to study the transcriptional response of the human intestinal cell line Caco-2 to rotavirus infection. We found that 508 genes were differentially regulated >2-fold at 16 h after rotavirus infection, and only one gene was similarly regulated at 1 h postinfection. Of these transcriptional changes, 73% corresponded to the upregulation of genes, with the majority of them occurring late, at 12 or more hours postinfection. Some of the regulated genes were classified according to known biological function and included genes encoding integral membrane proteins, interferon-regulated genes, transcriptional and translational regulators, and calcium metabolism-related genes. A new ...Continue Reading

References

Jul 1, 1977·Journal of the National Cancer Institute·J FoghT Orfeo
Mar 1, 1991·The Journal of General Virology·J R del CastilloF Liprandi
Mar 1, 1988·Journal of Pediatric Gastroenterology and Nutrition·D J Keljo, A K Smith
May 1, 1988·Journal of Medical Virology·R L Heath, C J Birch
Oct 15, 1983·International Journal of Cancer. Journal International Du Cancer·A ZweibaumK Haffen
Jan 1, 1993·Journal of Pediatric Gastroenterology and Nutrition·D De BoissieuC Dupont
Apr 5, 1996·Science·R I GlassB Ivanoff
Jun 1, 1996·Trends in Microbiology·R MénardP J Sansonetti
Apr 30, 1998·Journal of Virological Methods·V ChieuxP Wattré
Oct 6, 1998·Annual Review of Biochemistry·G R StarkR D Schreiber
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·H ZhuT Shenk
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Aug 5, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J AngelD Bass
Oct 27, 1999·Journal of Medical Virology·V ChieuxP Wattre
Aug 11, 2000·Seminars in Cancer Biology·G C Sen
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·C A GuerreroC F Arias

❮ Previous
Next ❯

Citations

Apr 8, 2004·Virus Research·Carlos F AriasSusana López
Apr 5, 2005·Nature Reviews. Microbiology·Richard G Jenner, Richard A Young
Apr 15, 2011·The Journal of Biological Chemistry·Dipanjan DuttaMamta Chawla-Sarkar
Nov 25, 2005·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Chu-Wen YangChen-Kung Chou
May 14, 2009·Viral Immunology·Luz-Stella RodríguezJuana Angel
Nov 22, 2005·Infection and Immunity·Katarina Roxström-LindquistStaffan Svärd
Apr 4, 2008·Journal of Virology·Liliana Maruri-AvidalCarlos F Arias
Aug 21, 2002·Journal of Virology·Joel W GraffMichele E Hardy
Nov 25, 2005·Journal of Virology·Dang Thi LuaTakashi Aoki
May 3, 2013·The Journal of Clinical Investigation·Emily M DealHarry B Greenberg
Jul 22, 2011·Virology Journal·Jose Luis ZambranoMichele E Hardy
Jan 18, 2005·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Stefania PiersantiIsabella Saggio
Dec 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brunella TaddeoBernard Roizman
Mar 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mario Barro, John T Patton
Oct 18, 2013·Journal of Virology·Adrish SenHarry B Greenberg
Jan 12, 2008·Journal of Virological Methods·Radhey S KaushikPhilip Griebel
Sep 25, 2014·Pharmacotherapy·Carlos A GuerreroOrlando Acosta
Jul 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Neung-Seon SeoMary K Estes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.