Gene Expression Pattern of PI3K/AKT Pathway Based on Insulin Resistance and Vitamin D Toward the Understanding of T2DM Pathogenesis

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Somayeh Alsadat Hosseini Khoramihuzwah Khaza’ai

Abstract

Background Insulin-stimulated glucose transport occurs via PI3K/AKT-dependent pathway which results in GLUT4 translocation from intracellular vesicles to plasma membrane and glucose uptake. PTEN, as a phosphatase, is the main antagonist of the PI3K/AKT pathway’s kinases. Present study was performed to investigate underlying mechanism responsible for defects in insulin signaling and the plausible role of vitamin D in pathogenesis of T2DM through the comparison of serum vitamin D and expression of genes involved in insulin signaling in diabetics and non-diabetics.Results RT-PCR was employed to investigate mRNA expression level of IRS1/PI3K/PDK1/AKT2/GLUT4/PTEN in diabetic and non-diabetic participants and serum vitamin D was measured by HPLC. Findings provide evidence that IRS1 expression was preserved while PI3K /AKT2/GLUT4 were expressed significantly lower and PDK1 was expressed significantly higher in diabetics compared to non-diabetics. Albeit there was no significant difference in PTEN expression between groups, it was up-regulated by the years of having diabetes. No significant correlation between serum vitamin D and gene expression of GOIs was observed in either group.Conclusion Since PTEN has been identified as a negativ...Continue Reading

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