Gene expression patterns and catalytic properties of UDP-D-glucose 4-epimerases from barley (Hordeum vulgare L.)

The Biochemical Journal
Qisen ZhangGeoffrey B Fincher

Abstract

UGE (UDP-Glc 4-epimerase or UDP-Gal 4-epimerase; EC 5.1.3.2) catalyses the interconversion of UDP-Gal and UDP-Glc. Both nucleotide sugars act as activated sugar donors for the biosynthesis of cell wall polysaccharides such as cellulose, xyloglucans, (1,3;1,4)-beta-D-glucan and pectins, together with other biologically significant compounds including glycoproteins and glycolipids. Three members of the HvUGE (barley UGE) gene family, designated HvUGE1, HvUGE2 and HvUGE3, have been characterized. Q-PCR (quantitative real-time PCR) showed that HvUGE1 mRNA was most abundant in leaf tips and mature roots, but its expression levels were relatively low in basal leaves and root tips. The HvUGE2 gene was transcribed at significant levels in all organs examined, while HvUGE3 mRNA levels were very low in all the organs. Heterologous expression of a near full-length cDNA confirmed that HvUGE1 encodes a functional UGE. A non-covalently bound NAD+ was released from the enzyme after denaturing with aqueous ethanol and was identified by its spectrophotometric properties and by electrospray ionization MS. The K(m) values were 40 microM for UDP-Gal and 55 muM for UDP-Glc. HvUGE also catalyses the interconversion of UDP-GalNAc and UDP-GlcNAc, alth...Continue Reading

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Citations

Mar 5, 2011·Annual Review of Plant Biology·Maor Bar-Peled, Malcolm A O'Neill
Mar 26, 2011·Plant Physiology and Biochemistry : PPB·Wioleta WojtasikJan Szopa
Jan 1, 2020·Journal of Asian Natural Products Research·Yan YangWei Wang
Apr 29, 2006·The Journal of Biological Chemistry·Christine BarberGeorg J Seifert
Oct 4, 2017·Frontiers in Plant Science·Daniel Decker, Leszek A Kleczkowski
Feb 15, 2019·International Journal of Molecular Sciences·Tingting JiaFuqiang Song
Jun 8, 2007·Biochimica Et Biophysica Acta·Shivani AgarwalAparna Dixit

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