Gene expression patterns for GDNF and its receptors in the human putamen affected by Parkinson's disease: a real-time PCR study

Molecular and Cellular Endocrinology
Cristina M BäckmanA Tomac

Abstract

Glial cell line-derived neurotrophic factor (GDNF), a member of the transforming growth factor-beta superfamily, is a potent trophic factor for dopaminergic neurons of the ventral midbrain, which are known to degenerate during Parkinson's disease (PD). The neuroprotective, neurorestorative, and stimulatory properties of GDNF has prompted numerous suggestions that this trophic factor may be a potential therapeutic tool to treat PD, and it has also been widely speculated that altered GDNF expression levels may be involved in the pathophysiology of the disease. In this study, we have investigated if mRNA expression levels for GDNF and/or its receptors are altered during PD in the human putamen, a target area for dopamine neurons of the substantia nigra compacta. Expression levels were analyzed with quantitative real-time reverse transcriptase polymerase reaction (RT qPCR) in post-mortem tissues from PD patients and aged matched controls. Primer pairs specific for GDNF (isoforms I and II), and its receptor molecules, GFRalpha1 and cRET were utilized. GDNF, cRET and GFRalpha1 mRNA expression was clearly detected in the putamen of control and Parkinson's disease patients. A modest but significant upregulation of GDNF mRNA levels (Iso...Continue Reading

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References

May 15, 1995·The Journal of Comparative Neurology·K E BowenkampA C Granholm
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·A TomacL Olson
Mar 21, 1996·Nature·D M GashG A Gerhardt
Jul 4, 1996·Nature·J J TreanorA Rosenthal
Feb 7, 1997·Science·D L Choi-LundbergM C Bohn
Jan 5, 2000·Journal of Biotechnology·O ThellinE Heinen
Oct 19, 2001·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·K GorzelniakA M Sharma
Apr 16, 2003·Journal of Neurochemistry·Matthew R HyndPeter R Dodd
Oct 16, 2003·Brain Research. Molecular Brain Research·Amanda D SmithMark C Austin
Jan 5, 2005·Experimental Neurology·Timothy J CollierJeffrey H Kordower

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Citations

Sep 4, 2012·Journal of Applied Genetics·Dimitry A ChistiakovVladimir P Chekhonin
Apr 9, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Amie L Peterson, John G Nutt
Sep 21, 2011·Nature Reviews. Neurology·Barry J Hoffer, Brandon K Harvey
Oct 22, 2015·Molecular Human Reproduction·S StreiterR Abir
Aug 12, 2015·Antioxidants & Redox Signaling·Angel Martín de PablosEmilio Fernández
Aug 15, 2014·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Shuqin CuiChao Zhu
Dec 15, 2010·Neurobiology of Aging·Ofelia M LittrellHeather A Boger
Nov 27, 2007·Journal of Neurochemistry·Ashley D ReynoldsHoward E Gendelman
Nov 11, 2010·EMBO Molecular Medicine·Xiaoli GuoTakayuki Harada
Aug 23, 2019·NMR in Biomedicine·Lydia M Le PageMyriam M Chaumeil
Apr 9, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rona BaronAlon Monsonego
Sep 2, 2020·Cell and Tissue Research·James A ConwayEdgar R Kramer
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Henrik WilmsRalph Lucius
Jun 23, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alberto FerriMaria Teresa Carrì
Jul 30, 2020·Cell and Tissue Research·Pia Runeberg-Roos, Richard D Penn
Dec 23, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Asparouh I IlievHarald Neumann
Mar 20, 2012·Neurologia i neurochirurgia polska·Jarosław Sławek
Apr 8, 2019·Human Genetics·Solenne CorreardCatherine André
Sep 22, 2020·Brain Communications·Daniel Enterría-MoralesXavier d'Anglemont de Tassigny
Apr 22, 2016·The European Journal of Neuroscience·Juliann D JaumotteMichael J Zigmond
Aug 20, 2008·Journal of Neuroscience Research·Naomi L CookCorinna van den Heuvel
Oct 31, 2015·Brain and Behavior·Laura K K PaceyDavid R Hampson
Sep 23, 2014·Aging Cell·Tianyi Jiang, Enrique Cadenas
Oct 20, 2020·Frontiers in Neuroscience·Raygene Martier, Pavlina Konstantinova
Apr 26, 2021·Trends in Molecular Medicine·Danqing ZhuDavid V Schaffer
Jul 3, 2021·International Journal of Molecular Sciences·Ana Fajardo-SerranoJosé Luis Lanciego
Oct 1, 2008·Progress in Neurobiology·Ana SaavedraEmília P Duarte
Mar 30, 2010·Journal of Chemical Neuroanatomy·Phalguni Anand AlladiUday Muthane

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