Gene expression patterns of novel visual and non-visual opsin families in immature and mature Japanese eel males

PeerJ
Jun-Hwan ByunSung-Pyo Hur

Abstract

This study was carried out to identify and estimate physiological function of a new type of opsin subfamily present in the retina and whole brain tissues of Japanese eel using RNA-Seq transcriptome method. A total of 18 opsin subfamilies were identified through RNA-seq. The visual opsin family included Rh2, SWS2, FWO, DSO, and Exo-Rhod. The non-visual opsin family included four types of melanopsin subfamily (Opn4x1, Opn4x2, Opn4m1, and Opn4m2), peropsin, two types of neuropsin subfamily (Opn5-like, Opn5), Opn3, three types of TMT opsin subfamily (TMT1, 2, 3), VA-opsin, and parapinopsin. In terms of changes in photoreceptor gene expression in the retina of sexually mature and immature male eels, DSO mRNA increased in the maturation group. Analysis of expression of opsin family gene in male eel brain before and after maturation revealed that DSO and SWS2 expression in terms of visual opsin mRNA increased in the sexually mature group. In terms of non-visual opsin mRNA, parapinopsin mRNA increased whereas that of TMT2 decreased in the fore-brain of the sexually mature group. The mRNA for parapinopsin increased in the mid-brain of the sexually mature group, whereas those of TMT1 and TMT3 increased in the hind-brain of the sexually m...Continue Reading

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Citations

Sep 26, 2021·Molecular Biology and Evolution·Nik LupšeZuzana Musilova

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Methods Mentioned

BETA
RNA-Seq
chip
electrophoresis
PCR

Software Mentioned

Primer3
cd
Primer Biosoft
tBlastn
tophat2
GraphPad Prism
HIT
RAxML
Bowtie2
PRINSEQ lite

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