Gene expression profile activated by the chemokine CCL5/RANTES in human neuronal cells

Journal of Neuroscience Research
Alessandra ValerioPierfranco Spano

Abstract

Differentiated human NT2-N neurons were shown to express CCR5 and CXCR4 chemokine receptor mRNA and protein, and to be responsive to the chemokines CCL5 and CXCL12. Using cDNA microarray technology, CCL5 was found to induce a distinct transcriptional program, with reproducible induction of 46 and 9 genes after 2 and 8 hr of treatment, respectively. Conversely, downregulation of 20 and 7 genes was observed after 2 and 8 hr of treatment, respectively. Modulation of a selected panel of CCL5-responsive genes was also confirmed by quantitative RT-PCR and Western blot and compared to gene expression changes induced by CXCL12 treatment. Gene clustering identified distinct functional subsets of CCL5-responsive molecules, and a significant number of expressed sequence tags encoding unknown genes. CCL5-responsive genes comprise a significant number of enzymes, transcription factors, and miscellaneous molecules involved in neuronal survival and differentiation, including neurite outgrowth and synaptogenesis. Our results suggest that CCL5 biological functions might go beyond its recognized chemotactic activity in the central nervous system, in particular with regard to the control of neural plasticity events both during development and in ...Continue Reading

References

Apr 20, 1995·Nature·T ChittendenB C Guild
Apr 1, 1998·Journal of Neuroimmunology·L M BolinK B Bacon
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Q MaT A Springer
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·O MeucciR J Miller
Apr 2, 1999·Trends in Pharmacological Sciences·F MennickenR Quirion
Apr 6, 1999·Journal of Neurovirology·A R Glabinski, R M Ransohoff
Jun 4, 1999·Immunology Today·A Mantovani
Oct 26, 1999·Trends in Neurosciences·V C Asensio, I L Campbell
Feb 23, 2000·Journal of Neuroimmunology·K B Bacon, J K Harrison
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R BonecchiS Sozzani
Mar 29, 2001·Nature Immunology·B Moser, P Loetscher
Jun 29, 2001·Mechanisms of Development·C Lambert de Rouvroit, A M Goffinet
Jul 31, 2001·Nature Neuroscience·N InagakiK Kaibuchi
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·S M McCormickK K Chittur
Jan 12, 2002·Current Opinion in Pharmacology·Knut BiberHendrikus W G M Boddeke
Feb 21, 2002·Trends in Cell Biology·Deanna S Smith, Li Huei Tsai
Mar 7, 2002·Journal of Neuroimmunology·Seog Bae OhRichard J Miller
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Massimo LocatiAlberto Mantovani
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Meiling LuRichard J Miller
Jun 18, 2002·Journal of Neurochemistry·Wing-Yu FuNancy Y Ip
Oct 2, 2002·Results and Problems in Cell Differentiation·Katrin I Andreasson, Walter E Kaufmann
Oct 31, 2002·European Journal of Human Genetics : EJHG·Bärbel FelderManuela C Koch
Nov 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Kevin BaconUNKNOWN IUIS/WHO Subcommittee on Chemokine Nomenclature
Apr 23, 2003·The Journal of Cell Biology·Yoshiki ArakawaShuh Narumiya
May 6, 2003·Science·Majid HafezparastElizabeth M C Fisher
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Domna Karagogeos

❮ Previous
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Citations

Jun 16, 2012·Cell and Tissue Research·Anne Jaerve, Hans Werner Müller
Oct 22, 2009·American Journal of Respiratory Cell and Molecular Biology·Maud M GuedersDidier D Cataldo
Mar 16, 2007·BMC Neuroscience·Andreas R PfenningAlison L Barth
Feb 3, 2016·Stem Cell Research & Therapy·Saeed AlghamdiG Rasul Chaudhry
Mar 27, 2012·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Milan FialaMartin Weinand
Dec 21, 2010·Neurochemistry International·Chun-Jung ChenShih-Yun Chen
Jun 13, 2008·Journal of Neuroimmunology·Alice GuyonJean-Louis Nahon
Apr 29, 2008·Neurobiology of Aging·Debjani TripathyPaula Grammas
Apr 21, 2006·The European Journal of Neuroscience·Alessandra ValerioMarina Pizzi
Aug 22, 2006·Brain Research·Elisabeth FrøylandTerje Rootwelt
Oct 16, 2012·Brain Research Bulletin·Guilin LiShangdong Liang
Mar 17, 2015·Cellular and Molecular Life Sciences : CMLS·Sadat DokanehiifardSeyed Javad Mowla
Sep 8, 2016·Behavioural Brain Research·Thomas R GrovesAntiño R Allen
Mar 30, 2017·Scientific Reports·Gary Hin-Fai YamJodhbir S Mehta
Jan 7, 2016·Multiple Sclerosis : Clinical and Laboratory Research·Francesco MoriDiego Centonze
Jan 18, 2019·Viruses·James A Carroll, Bruce Chesebro
Oct 11, 2020·American Journal of Perinatology·Athina PappasRosemary D Higgins
Apr 4, 2021·Biomolecules·Leonor OrgeMaria Dos Anjos Pires

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