Gene expression profile analysis of aortic vascular smooth muscle cells reveals upregulation of cadherin genes in myocardial infarction patients

Physiological Genomics
Anselm A DerdaVitaly A Sorokin

Abstract

Myocardial infarction (MI) induced by acute coronary arterial occlusion is usually secondary to atherosclerotic plaque rupture. Dysregulated response of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques may promote plaque rupture. Cadherins (CDHs) form adherens junctions and are known stabilizers of atherosclerotic plaques. To date, the expression patterns of cadherin have not been well investigated in MI aortic VSMCs. We aimed to investigate the expression of cadherin genes in the aortic wall of patients with and without MI. Laser capture microdissected VSMCs were obtained from aortic tissue samples of patients undergoing coronary artery bypass graft surgery. Integrative bioinformatic analysis of the microarray profiles of the VSMCs revealed that MI is discriminated at the whole transcriptome level by hundreds of differentially expressed genes, including genes involved in cell adhesion, of which the cadherin superfamily genes were among the top structural category. Eleven significantly deregulated candidates of the cadherin superfamily were chosen and formed a new classifier that collectively discriminated MI vs. non-MI with ~95% accuracy. Significance validation was performed with an independent cohort by quanti...Continue Reading

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Citations

Mar 1, 2020·International Journal of Molecular Sciences·Chien-Lin LuYee-Yung Ng
Jun 3, 2020·Journal of Cellular and Molecular Medicine·Jae Gyun OhDongtak Jeong
Jan 18, 2020·International Journal of Molecular Sciences·Branislav KuraJan Slezak
Sep 25, 2019·International Journal of Molecular Sciences·Nadine WehbeElias Baydoun
Aug 8, 2019·International Journal of Molecular Sciences·Louise E See HoeJohn F Fraser

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Datasets Mentioned

BETA
GSE83500

Methods Mentioned

BETA
coronary artery bypass
laser capture microdissection
PCR
dissection
PCA

Software Mentioned

limma
SPSS
AmiGO2
Sytel Studio
R “ limma
InterPro
Sytel
UP
Feature
R package

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