Gene expression profile changes correlating with radioresistance in human cell lines

International Journal of Radiation Oncology, Biology, Physics
Ken-ichi IshikawaTakashi Imai

Abstract

To identify gene expression profiles specific to radioresistance of human cells. Global gene expression profiles of a total of 15 tumor and normal fibroblast cell lines were analyzed using DNA microarrays and statistical clustering methods. Initially, six of the cell lines were categorized into radioresistant (RG) or nonradioresistant (NRG) groups according to the radiation dose required to reduce their survival to 10% (D10). Genes for which expression was specific to each group at 1 or 3 h after irradiation were identified using statistical procedures including analysis of variance and a two-dimensional hierarchical clustering method. The remaining nine cell lines were subjected to the k-nearest neighbor pattern classification. The nine test cell lines were successfully classified by their D10 value using 46 and 44 genes for which transcription levels had significantly changed at 1 and 3 h after irradiation, respectively. Of these genes, 25 showed altered expression at both time points in the NRG or RG, but independently were unable to classify the test cell lines. Radioresistant cell lines analyzed in this study showed certain radiation-induced changes in gene expression profiles that are different from the profile changes of...Continue Reading

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Citations

Feb 26, 2014·International Journal of Molecular Sciences·Ken-ichi IshikawaTakashi Imai
Sep 4, 2009·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·H Peter Rodemann
Apr 1, 2008·International Journal of Radiation Oncology, Biology, Physics·Tomo SugaTakashi Imai
Jan 12, 2008·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·Christophe HennequinVincent Favaudon
Sep 25, 2007·International Journal of Radiation Oncology, Biology, Physics·Tomo SugaTakashi Imai
May 22, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jan AlsnerJens Overgaard
Jun 28, 2012·Genes, Chromosomes & Cancer·Aik Kia KhawM Prakash Hande
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Oct 14, 2014·Molecular Cancer·Resham Lal GurungM Prakash Hande
Oct 30, 2007·Biochemical and Biophysical Research Communications·Hitomi SudoYoshi-nobu Harada
Apr 23, 2019·Radiation Research·Hakan OestenArmin Lühr
Mar 9, 2020·Radiation and Environmental Biophysics·K E ApplegateS Bouffler
Nov 11, 2008·Experimental Biology and Medicine·Kazunori NojiriTakashi Imai

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