Gene expression profile of adult human bone marrow-derived mesenchymal stem cells stimulated by the chemokine CXCL7

The International Journal of Biochemistry & Cell Biology
G KalwitzChristian Kaps

Abstract

A variety of chemokines has been shown to recruit human bone marrow-derived mesenchymal stem cells (MSC) and may be potential candidates for chemokine-based tissue regeneration approaches. The aim of our study was to determine whether the chemokine CXCL7 stimulates migration of human bone marrow-derived MSC and to analyze the effect of CXCL7 on the recruitment of MSC on the broad molecular level. Chemotaxis assays documented that high doses of CXCL7 significantly recruited MSC. Gene expression profiling using oligonucleotide microarrays showed that MSC treated with CXCL7 differentially expressed genes related to cell migration, cell adhesion and extracellular matrix remodeling. Pathway analysis showed that CXCL7 induced the expression of all chemokines binding the interleukin (IL) receptors A and B, CXCR1 and CXCR2, as well as the IL6 signal transducer (gp130) and its ligands IL6 and leukemia inhibitory factor (LIF). Induction of differentially expressed chemokines CXCL1-3, CXCL5, and CXCL6 as well as LIF and gp130 in MSC by CXCL7 was verified by real-time polymerase chain reaction. Immunoassay of cell culture supernatants confirmed elevated levels of the interleukins 6 and 8 in MSC upon treatment with CXCL7. Chemotaxis assays ...Continue Reading

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Citations

Oct 13, 2010·Journal of Molecular Neuroscience : MN·Naghmeh Ahmadian KiaHamid Reza Bidkhori
Apr 13, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Apr 25, 2013·The International Journal of Biochemistry & Cell Biology·Rebecca KulawigMichaela Endres
Jul 24, 2010·Journal of Tissue Engineering and Regenerative Medicine·Gregor KalwitzChristian Kaps
Oct 12, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Juan BayoGuillermo Mazzolini
Apr 8, 2016·Stem Cell Reports·Catarina R AlmeidaMário A Barbosa
May 11, 2010·Genomics·Margaret C Emblom-CallahanSteven D Nathan
Aug 29, 2016·Stem Cell Research & Therapy·Vitali AlexeevOlga Igoucheva
Jan 29, 2011·Spine·Aldemar A HegewaldClaudius Thomé
Mar 20, 2009·Cell and Tissue Research·Stefan StichJochen Ringe
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·A CerqueiraJ Suay
Feb 9, 2018·Biomaterials·Stephen KennedyDavid Mooney

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