Gene expression profile of neuronal progenitor cells derived from hESCs: activation of chromosome 11p15.5 and comparison to human dopaminergic neurons

PloS One
William J FreedXianmin Zeng

Abstract

We initiated differentiation of human embryonic stem cells (hESCs) into dopamine neurons, obtained a purified population of neuronal precursor cells by cell sorting, and determined patterns of gene transcription. Dopaminergic differentiation of hESCs was initiated by culturing hESCs with a feeder layer of PA6 cells. Differentiating cells were then sorted to obtain a pure population of PSA-NCAM-expressing neuronal precursors, which were then analyzed for gene expression using Massive Parallel Signature Sequencing (MPSS). Individual genes as well as regions of the genome which were activated were determined. A number of genes known to be involved in the specification of dopaminergic neurons, including MSX1, CDKN1C, Pitx1 and Pitx2, as well as several novel genes not previously associated with dopaminergic differentiation, were expressed. Notably, we found that a specific region of the genome located on chromosome 11p15.5 was highly activated. This region contains several genes which have previously been associated with the function of dopaminergic neurons, including the gene for tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, IGF2, and CDKN1C, which cooperates with Nurr1 in directing the differe...Continue Reading

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Citations

Apr 24, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jenica D TapocikNorman H Lee
Apr 17, 2010·Neuroscience·Z VuceticT M Reyes
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Jan 5, 2013·Journal of Proteome Research·Kyung-Hoon KwonJong Shin Yoo

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Datasets Mentioned

BETA
BC000531

Methods Mentioned

BETA
flow cytometry
transgenic
dissection
PCR
Fluorescence
FACS
laser capture microdissection

Software Mentioned

pClamp
MPSS

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