Gene Expression Profile of the Human Colorectal Carcinoma LoVo Cells Treated With Sporamin and Thapsigargin.

Frontiers in Oncology
Chun YangPeng-Gao Li

Abstract

Sporamin, a proteinase inhibitor isolated from the sweet potato (Ipomoea batatas), has shown promising anticancer effect against colorectal cancer (CRC) in vitro and in vivo but its mechanisms of action are poorly understood. In the present study, high throughput RNA sequencing (RNA-seq) technology was applied to explore the transcriptomic changes induced by sporamin in the presence of thapsigargin (TG), a non-12-O-tetradecanolphorbol-13-acetate type cancer promoter, in the LoVo human CRC cells. Cellular total RNA was extracted from the cells after they were treated with vehicle (CTL), 1 μM of thapsigargin (TG), or 1 μM of TG plus 30 μM of sporamin (TGSP) for 24 h. The migratory capacity of the cells was determined by wound healing assay. The gene expression profiles of the cells were determined by RNA-seq on an Illumina platform. GO enrichment analysis, KEGG pathway analysis, protein-protein interaction (PPI) network construction, and transcription factors (TF) prediction were all performed based on the differentially expressed genes (DEGs) across groups with a series of bioinformatics tools. Finally, the effect and potential molecular targets of the sporamin at the transcriptome level were evaluated. Sporamin significantly in...Continue Reading

References

Mar 1, 1991·The Journal of Prosthetic Dentistry·B J CrispinH Globe
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Nov 1, 1989·British Journal of Pharmacology·D J BraydenA W Cuthbert
Aug 2, 2001·Biochemical and Biophysical Research Communications·T G PaiM C Liu
Jun 8, 2004·Bioinformatics·Alok J Saldanha
Apr 9, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elizabeth A KiddHoward L McLeod
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Dec 14, 2007·Nucleic Acids Research·Minoru KanehisaYoshihiro Yamanishi
Dec 18, 2007·Bioinformation·Lokesh Kumar, Matthias E Futschik
Aug 15, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Zoe RichesMichael W H Coughtrie
Dec 19, 2009·The New England Journal of Medicine·Sanford D Markowitz, Monica M Bertagnolli
Apr 23, 2010·Fundamental & Clinical Pharmacology·Jun Yao, Cuijuan Qian
Jul 27, 2011·Therapeutic Advances in Medical Oncology·Michael S Braun, Matthew T Seymour
Jun 8, 2013·World Journal of Gastroenterology : WJG·Peng-Gao LiLe Deng
Mar 1, 2014·The Journal of Surgical Research·Hongwei ChenHongying Qiao
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Nov 18, 2014·Nature Methods·Benjamin BuchfinkDaniel H Huson
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
May 15, 2015·Nature·Herb Brody
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yoji MurataTakashi Matozaki
Jan 12, 2016·Therapeutic Advances in Medical Oncology·William A HammondKabir Mody
Jan 28, 2016·Regenerative Biomaterials·Juan LiAiguo Wu
Mar 18, 2017·International Journal of Molecular Sciences·Thazin Nwe AungDavid L Adelson
Jul 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Cuijuan QianJun Yao
Nov 30, 2018·International Journal of Molecular Sciences·Teodora CosteaMaria-Magdalena Mocanu
Mar 31, 2019·Journal of Hematology & Oncology·Yanhua MouBin Li
Jun 6, 2019·International Journal of Molecular Sciences·Giuseppe Antonio MalfaClaudia Di Giacomo

❮ Previous
Next ❯

Software Mentioned

Cytoscape
RSEM
EMBOSS
NIH ImageJ
R
Mfuzz
Cluster Treeview
WEGO
orf
DEGseq

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.