Gene expression profiles and intracellular contents of stress protectants in Saccharomyces cerevisiae under ethanol and sorbitol stresses

Applied Microbiology and Biotechnology
Tomohiro Kaino, Hiroshi Takagi

Abstract

In response to osmotic stress, proline is accumulated in many bacterial and plant cells. During various stresses, the yeast Saccharomyces cerevisiae induces glycerol or trehalose synthesis, but the fluctuations in gene expression and intracellular levels of proline in yeast are not yet well understood. We previously found that proline protects yeast cells from damage by freezing, oxidative, or ethanol stress. In this study, we examined the relationships between the gene expression profiles and intracellular contents of glycerol, trehalose, and proline under stress conditions. When yeast cells were exposed to 1 M sorbitol stress, the expression of GPD1 encoding glycerol-3-phosphate dehydrogenase is induced, leading to glycerol accumulation. In contrast, in the presence of 9% ethanol, the rapid induction of TPS2 encoding trehalose-6-phosphate phosphatase resulted in trehalose accumulation. We found that intracellular proline levels did not increase immediately after addition of sorbitol or ethanol. However, the expressions of genes involved in proline synthesis and degradation did not change during exposure to these stresses. It appears that the elevated proline levels are due primarily to an increase in proline uptake from a nut...Continue Reading

References

Dec 1, 1987·Journal of Bacteriology·D M Tomenchok, M C Brandriss
Jul 1, 1986·Molecular and Cellular Biology·S S Wang, M C Brandriss
Oct 1, 1983·Molecular and Cellular Biology·M C Brandriss
Oct 1, 1981·Journal of Bacteriology·P F Lasko, M C Brandriss
May 11, 1994·Biochimica Et Biophysica Acta·J J MansureJ H Crowe
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·N VerbruggenM Van Montagu
Nov 27, 1998·Trends in Biotechnology·M A Singer, S Lindquist
Dec 5, 1998·The Journal of Biological Chemistry·W BellJ M Thevelein
Mar 27, 1999·Journal of Molecular Evolution·L Aravind, E V Koonin
Mar 15, 2000·Protein Science : a Publication of the Protein Society·D SamuelC Yu
Feb 17, 2001·Molecular Biology of the Cell·H C CaustonR A Young
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 26, 2003·FEBS Letters·Adriana Garay-ArroyoAlejandra A Covarrubias
Dec 9, 1964·Biochimica Et Biophysica Acta·W J MIDDELHOVEN
Dec 4, 2003·Environmental Microbiology·John E HallsworthKenneth N Timmis
Dec 9, 2003·Applied and Environmental Microbiology·John E HallsworthKenneth N Timmis
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Changbin Chen, Martin B Dickman
Oct 20, 2005·Journal of Bioscience and Bioengineering·Yuko MoritaHiroshi Takagi
Dec 8, 2005·Applied and Environmental Microbiology·Hiroshi TakagiYoshito Kubo

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Citations

May 14, 2010·Applied Microbiology and Biotechnology·Menggen Ma, Z Lewis Liu
Mar 31, 2010·The Journal of Biological Chemistry·Iwao OhtsuHiroshi Takagi
Oct 20, 2010·Omics : a Journal of Integrative Biology·Ming-Zhu DingYing-Jin Yuan
Jul 22, 2008·Applied and Environmental Microbiology·Tomohiro KainoHiroshi Takagi
Aug 5, 2009·Infection and Immunity·Popchai NgamskulrungrojJohn R Perfect
Mar 28, 2012·Bioscience, Biotechnology, and Biochemistry·Tomohiro KainoHiroshi Takagi
Feb 1, 2014·Applied Biochemistry and Biotechnology·Xiaojian ShiJining Wang
Jan 9, 2013·Journal of Bioscience and Bioengineering·Yosuke KobayashiTakafumi Kasumi
Nov 2, 2011·International Journal of Food Microbiology·Yu SasanoHiroshi Takagi
May 6, 2011·Journal of Bioscience and Bioengineering·Francisco B PereiraLucília Domingues
Jan 23, 2013·Microbial Biotechnology·Jonathan A CrayJohn E Hallsworth
Aug 11, 2016·Scientific Reports·Yanfei ChengXiuping He
Jun 7, 2017·FEMS Yeast Research·Quinten DeparisJohan M Thevelein
Mar 13, 2018·The Journal of General and Applied Microbiology·Hao JiBin Zhuge
Oct 3, 2014·The Journal of General and Applied Microbiology·Nobushige NakazawaKeitaro Takahashi
Mar 23, 2017·Frontiers in Microbiology·Elisabet Navarro-TapiaAmparo Querol
Aug 22, 2018·Applied Microbiology and Biotechnology·Jianmei LuoMin Wang
Jan 3, 2019·Antonie van Leeuwenhoek·Mariana Caroline Tocantins AlvimWendel Batista da Silveira
May 16, 2017·Biotechnology for Biofuels·Gumpanat MahipantAlisa S Vangnai
Dec 3, 2020·Frontiers in Genetics·Caitlin LahueCaiti Smukowski Heil

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