Gene expression profiles characterize inflammation stages in the acute lung injury in mice.

PloS One
Isabelle LesurCatherine Nguyen

Abstract

Acute Lung Injury (ALI) carries about 50 percent mortality and is frequently associated with an infection (sepsis). Life-support treatment with mechanical ventilation rescues many patients, although superimposed infection or multiple organ failure can result in death. The outcome of a patient developing sepsis depends on two factors: the infection and the pre-existing inflammation. In this study, we described each stage of the inflammation process using a transcriptional approach and an animal model. Female C57BL6/J mice received an intravenous oleic acid injection to induce an acute lung injury (ALI). Lung expression patterns were analyzed using a 9900 cDNA mouse microarray (MUSV29K). Our gene-expression analysis revealed marked changes in the immune and inflammatory response metabolic pathways, notably lipid metabolism and transcription. The early stage (1 hour-1.5 hours) is characterized by a pro-inflammatory immune response. Later (3 hours-4 hours), the immune cells migrate into inflamed tissues through interaction with vascular endothelial cells. Finally, at late stages of lung inflammation (18 hours-24 hours), metabolism is deeply disturbed. Highly expressed pro-inflammatory cytokines activate transcription of many genes ...Continue Reading

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Citations

Nov 8, 2012·Mediators of Inflammation·C F Gonçalves-de-AlbuquerqueM V Castro-Faria
Oct 25, 2011·Biomaterials·Juthamas RatanavarapornYasuhiko Tabata
Sep 14, 2016·Infection and Immunity·Wenlong ZhangYongguo Cao
Aug 21, 2019·Intensive Care Medicine Experimental·Thi Thom TranNathalie Lalevée
Nov 13, 2020·Redox Biology·Valentina GrippoJan Vacek
Aug 16, 2016·The American Journal of Pathology·Zanmei ZhaoWen Xie

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Datasets Mentioned

BETA
GSE18712

Methods Mentioned

BETA
PCR

Software Mentioned

Bzscan2
Microarrays Quality Control tool
Treeview
Cluster
SAM
SAM ( Analysis of Microarrays
Biocarta
DAVID
TIGR MeV ( MultiExperiment Viewer )

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