Gene expression profiles of circulating tumor cells versus primary tumors in metastatic breast cancer

Cancer Letters
Wendy OnstenkStefan Sleijfer

Abstract

Before using circulating tumor cells (CTCs) as liquid biopsy, insight into molecular discrepancies between CTCs and primary tumors is essential. We characterized CellSearch-enriched CTCs from 62 metastatic breast cancer (MBC) patients with ≥5 CTCs starting first-line systemic treatment. Expression levels of 35 tumor-associated, CTC-specific genes, including ESR1, coding for the estrogen receptor (ER), were measured by reverse transcription quantitative polymerase chain reaction and correlated to corresponding primary tumors. In 30 patients (48%), gene expression profiles of 35 genes were discrepant between CTCs and the primary tumor, but this had no prognostic consequences. In 15 patients (24%), the expression of ER was discrepant. Patients with ER-negative primary tumors and ER-positive CTCs had a longer median TTS compared to those with concordantly ER-negative CTCs (8.5 versus 2.1 months, P = 0.05). From seven patients, an axillary lymph node metastasis was available. In two patients, the CTC profiles better resembled the lymph node metastasis than the primary tumor. Our findings suggest that molecular discordances between CTCs and primary tumors frequently occur, but that this bears no prognostic consequences. Alterations i...Continue Reading

References

Apr 25, 2006·Biostatistics·W Evan JohnsonAriel Rabinovic
Oct 3, 2007·PLoS Genetics·Jeffrey T Leek, John D Storey
Jan 1, 2009·Journal of the National Cancer Institute·Anieta M SieuwertsJohn A Foekens
Jul 15, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·C LiedtkeA M Gonzalez-Angulo
Sep 15, 2010·Nature Reviews. Genetics·Jeffrey T LeekRafael A Irizarry
Nov 3, 2010·The Oncologist·Lajos PusztaiClifford A Hudis
Feb 24, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G CuriglianoA Goldhirsch
Apr 21, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anieta M SieuwertsJohn W M Martens
Nov 30, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Eitan AmirMark Clemons
Jun 20, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Linda Sofie LindströmJonas Bergh
Nov 20, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·F CardosoUNKNOWN ESMO Guidelines Working Group
Sep 25, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M V DieciV Guarneri
Sep 28, 2012·Clinical Chemistry·Catherine Alix-Panabières, Klaus Pantel

❮ Previous
Next ❯

Citations

Jan 27, 2016·Molecular Oncology·Francois-Clement BidardJean-Yves Pierga
Oct 21, 2016·Neoplasia : an International Journal for Oncology Research·Nick BeijeStefan Sleijfer
Apr 9, 2017·Genomics, Proteomics & Bioinformatics·Jinfeng Zou, Edwin Wang
Jan 24, 2019·Clinical Genetics·Sahar AlimirzaieMohammad R Akbari
Feb 15, 2019·International Journal of Cancer. Journal International Du Cancer·Ingeborg E de KruijffStefan Sleijfer
Jul 24, 2018·Oncotarget·Andreas JosefssonKarin Welén
Aug 16, 2019·Oncotarget·Diaddin HamdanGuilhem Bousquet
Sep 4, 2015·Current Opinion in Oncology·Isabelle GingrasMichail Ignatiadis
Aug 14, 2020·Breast Cancer Research : BCR·Afroditi NanouLeonardus Wendelinus Mathias Marie Terstappen
Aug 23, 2019·Biomicrofluidics·Florina S IliescuCiprian Iliescu
Apr 16, 2020·Advanced Biosystems·Semih CalamakUtkan Demirci
Nov 15, 2017·Molecular Cancer Therapeutics·Paul RiviereRazelle Kurzrock
Jan 23, 2018·Advanced Drug Delivery Reviews·Sabine RiethdorfKlaus Pantel
Oct 24, 2021·Clinical Genetics·Galo DuqueNayeli García

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.