Gene Expression Profiling and Assessment of Vitamin D and Serotonin Pathway Variations in Patients With Irritable Bowel Syndrome

Journal of Neurogastroenterology and Motility
Christopher M DussikPeter W Jurutka

Abstract

Irritable bowel syndrome (IBS) is a multifaceted disorder that afflicts millions of individuals worldwide. IBS is currently diagnosed based on the presence/duration of symptoms and systematic exclusion of other conditions. A more direct manner to identify IBS is needed to reduce healthcare costs and the time required for accurate diagnosis. The overarching objective of this work is to identify gene expression-based biological signatures and biomarkers of IBS. Gene transcripts from 24 tissue biopsy samples were hybridized to microarrays for gene expression profiling. A combination of multiple statistical analyses was utilized to narrow the raw microarray data to the top 200 differentially expressed genes between IBS versus control subjects. In addition, quantitative polymerase chain reaction was employed for validation of the DNA microarray data. Gene ontology/pathway enrichment analysis was performed to investigate gene expression patterns in biochemical pathways. Finally, since vitamin D has been shown to modulate serotonin production in some models, the relationship between serum vitamin D and IBS was investigated via 25-hydroxyvitamin D (25[OH]D) chemiluminescence immunoassay. A total of 858 genetic features were identified ...Continue Reading

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Citations

Feb 16, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Bruno Kotska Rodiño-JaneiroCristina Martínez
Mar 4, 2020·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Jae Soung LeeHyung-Kyoon Choi
Feb 23, 2019·Expert Review of Gastroenterology & Hepatology·Sandra Maria BarbalhoMarcelo Dib Bechara
Dec 26, 2018·Journal of Parkinson's Disease·Filip ScheperjansPer Borghammer
Jan 26, 2021·Database : the Journal of Biological Databases and Curation·Lauren ChanMelissa Haendel
Jan 29, 2021·Database : the Journal of Biological Databases and Curation·Lauren ChanMelissa Haendel
Jun 25, 2021·Journal of Cellular and Molecular Medicine·Sandra MohrBeate Niesler

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Methods Mentioned

BETA
Feature Extraction
PCR

Software Mentioned

Cytoscape
limma
Feature Extraction
ClueGO
R
Agilent
ReactomeFI

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