Gene expression profiling distinguishes between spontaneous and radiation-induced rat mammary carcinomas

Journal of Radiation Research
Tatsuhiko ImaokaYoshiya Shimada

Abstract

The ability to distinguish between spontaneous and radiation-induced cancers in humans is expected to improve the resolution of estimated risk from low dose radiation. Mammary carcinomas were obtained from Sprague-Dawley rats that were either untreated (n = 45) or acutely gamma-irradiated (1 Gy; n = 20) at seven weeks of age. Gene expression profiles of three spontaneous and four radiation-induced carcinomas, as well as those of normal mammary glands, were analyzed by microarrays. Differential expression of identified genes of interest was then verified by quantitative polymerase chain reaction (qPCR). Cluster analysis of global gene expression suggested that spontaneous carcinomas were distinguished from a heterogeneous population of radiation-induced carcinomas, though most gene expressions were common. We identified 50 genes that had different expression levels between spontaneous and radiogenic carcinomas. We then selected 18 genes for confirmation of the microarray data by qPCR analysis and obtained the following results: high expression of Plg, Pgr and Wnt4 was characteristic to all spontaneous carcinomas; Tnfsf11, Fgf10, Agtr1a, S100A9 and Pou3f3 showed high expression in a subset of radiation-induced carcinomas; and inc...Continue Reading

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Citations

Jun 10, 2009·Journal of Radiation Research·Tatsuhiko ImaokaYoshiya Shimada
Aug 4, 2010·Journal of Radiation Research·Nobuyuki HamadaTeruki Teshima
Jun 15, 2011·Biogerontology·Hagai YanaiVadim E Fraifeld
Jun 22, 2016·Endocrine-related Cancer·Cécile M VouyovitchHichem C Mertani
Oct 10, 2013·International Journal of Cancer. Journal International Du Cancer·Tatsuhiko ImaokaYoshiya Shimada
May 16, 2009·International Journal of Cancer. Journal International Du Cancer·Kazuhiro DainoSylvie Chevillard
Mar 12, 2016·International Journal of Radiation Biology·Tatsuhiko ImaokaShinji Yoshinaga

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