Gene Expression Profiling in Behcet's Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted Therapy

Journal of Immunology Research
Antonio PuccettiMarzia Dolcino

Abstract

Behçet disease (BD) is a chronic inflammatory multisystem disease characterized by oral and genital ulcers, uveitis, and skin lesions. Disease etiopathogenesis is still unclear. We aim to elucidate some aspects of BD pathogenesis and to identify specific gene signatures in peripheral blood cells (PBCs) of patients with active disease using novel gene expression and network analysis. 179 genes were modulated in 10 PBCs of BD patients when compared to 10 healthy donors. Among differentially expressed genes the top enriched gene function was immune response, characterized by upregulation of Th17-related genes and type I interferon- (IFN-) inducible genes. Th17 polarization was confirmed by FACS analysis. The transcriptome identified gene classes (vascular damage, blood coagulation, and inflammation) involved in the pathogenesis of the typical features of BD. Following network analysis, the resulting interactome showed 5 highly connected regions (clusters) enriched in T and B cell activation pathways and 2 clusters enriched in type I IFN, JAK/STAT, and TLR signaling pathways, all implicated in autoimmune diseases. We report here the first combined analysis of the transcriptome and interactome in PBCs of BD patients in the active st...Continue Reading

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Citations

Feb 16, 2020·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Sihem HouacineFarida Fortune
May 29, 2020·Annals of the Rheumatic Diseases·Jinjing LiuWenjie Zheng
Dec 19, 2019·Frontiers in Immunology·Farzan SolimaniKamran Ghoreschi
May 28, 2019·Frontiers in Immunology·Pietro Leccese, Erkan Alpsoy
Apr 14, 2020·International Immunopharmacology·Zichu ZhaoLingli Dong
Mar 18, 2021·Journal of Inflammation Research·Helal F HettaManal M Hassanien
Jul 20, 2020·Current Drug Targets·Jermilia CharlesVictoria A Ploplis
Sep 12, 2021·BMC Research Notes·Samuel J Shenoi, Erich J Baker

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Methods Mentioned

BETA
gene array
density-gradient centrifugation
FACS
Assay
ELISA
chip
flow cytometry
biopsies

Software Mentioned

PANTHER
Immunochip
STRING
Cytoscape
SPSS Statistics

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