Gene Expression Profiling in Fibromyalgia Indicates an Autoimmune Origin of the Disease and Opens New Avenues for Targeted Therapy

Journal of Clinical Medicine
Marzia DolcinoClaudio Lunardi

Abstract

Fibromyalgia is a chronic disorder characterized by widespread pain and by several non-pain symptoms. Autoimmunity, small fiber neuropathy and neuroinflammation have been suggested to be involved in the pathogenesis of the disease. We have investigated the gene expression profile in peripheral blood mononuclear cells obtained from ten patients and ten healthy subjects. Of the 545,500 transcripts analyzed, 1673 resulted modulated in fibromyalgic patients. The majority of these genes are involved in biological processes and pathways linked to the clinical manifestations of the disease. Moreover, genes involved in immunological pathways connected to interleukin-17 and to Type I interferon signatures were also modulated, suggesting that autoimmunity plays a role in the disease. We then aimed at identifying differentially expressed Long non-coding RNAs (LncRNAs) functionally connected to modulated genes both directly and via microRNA targeting. Only two LncRNAs of the 298 found modulated in patients, were able to target the most highly connected genes in the fibromyalgia interactome, suggesting their involvement in crucial gene regulation. Our gene expression data were confirmed by real time PCR, by autoantibody testing, detection o...Continue Reading

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Citations

Jan 7, 2021·Diagnostics·Rebeca Illescas-MontesJavier Ramos-Torrecillas
May 1, 2021·International Journal of Molecular Sciences·Rosalba SiracusaDaniela Impellizzeri

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Methods Mentioned

BETA
density-gradient centrifugation
fluorescence-activated cell sorting
chip
Assay
ELISA
dissection
gene

Software Mentioned

Thermofisher
Transcriptome Analysis Console ( TAC )
SPSS Statistics
FunRich
Panther
Cytoscape
STRING ( Search Tool for the Retrieval of Interacting Genes )

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

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