Gene expression profiling in necrotizing enterocolitis reveals pathways common to those reported in Crohn's disease

BMC Medical Genomics
Éric TremblayJean-François Beaulieu

Abstract

Necrotizing enterocolitis (NEC) is the most frequent life-threatening gastrointestinal disease experienced by premature infants in neonatal intensive care units. The challenge for neonatologists is to detect early clinical manifestations of NEC. One strategy would be to identify specific markers that could be used as early diagnostic tools to identify preterm infants most at risk of developing NEC or in the event of a diagnostic dilemma of suspected disease. As a first step in this direction, we sought to determine the specific gene expression profile of NEC. Deep sequencing (RNA-Seq) was used to establish the gene expression profiles in ileal samples obtained from preterm infants diagnosed with NEC and non-NEC conditions. Data were analyzed with Ingenuity Pathway Analysis and ToppCluster softwares. Data analysis indicated that the most significant functional pathways over-represented in NEC neonates were associated with immune functions, such as altered T and B cell signaling, B cell development, and the role of pattern recognition receptors for bacteria and viruses. Among the genes that were strongly modulated in neonates with NEC, we observed a significant degree of similarity when compared with those reported in Crohn's dis...Continue Reading

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Citations

Mar 21, 2017·Nitric Oxide : Biology and Chemistry·Emanuela FerrettiJean-François Beaulieu
Nov 12, 2016·Seminars in Perinatology·Alain Cuna, Venkatesh Sampath
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Nov 14, 2018·Gastroenterology Research and Practice·Laszlo MarkaszHelene Engstrand Lilja
Mar 6, 2021·BMC Research Notes·Éric TremblayJean-François Beaulieu
Apr 27, 2021·Expert Review of Proteomics·Lauren C BeckChristopher J Stewart
Jul 3, 2021·International Journal of Molecular Sciences·Luigia De FazioArianna Aceti
Sep 2, 2021·Pediatric Research·Marie-Pier ThibaultJean-François Beaulieu

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Datasets Mentioned

BETA
GSE64801

Methods Mentioned

BETA
RNA-Seq
RNASeq

Software Mentioned

IPA
TopHat for Illumina
ToppCluster
Cufflinks
Cuffdiff
pedCD
FastQC
RNASeq

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