Gene expression profiling in the inductive human hematopoietic microenvironment

Biochemical and Biophysical Research Communications
Yongjun ZhaoMargaret R Hough

Abstract

Human hematopoietic stem cells (HSCs) and their progenitors can be maintained in vitro in long-term bone marrow cultures (LTBMCs) in which constituent HSCs can persist within the adherent layers for up to 2 months. Media replenishment of LTBMCs has been shown to induce transition of HSCs from a quiescent state to an active cycling state. We hypothesize that the media replenishment of the LTBMCs leads to the activation of important regulatory genes uniquely involved in HSC proliferation and differentiation. To profile the gene expression changes associated with HSC activation, we performed suppression subtractive hybridization (SSH) on day 14 human LTBMCs following 1-h media replenishment and on unmanipulated controls. The generated SSH library contained 191 differentially up-regulated expressed sequence tags (ESTs), the majority corresponding to known genes related to various intracellular processes, including signal transduction pathways, protein synthesis, and cell cycle regulation. Nineteen ESTs represented previously undescribed sequences encoding proteins of unknown function. Differential up-regulation of representative genes, including IL-8, IL-1, putative cytokine 21/HC21, MAD3, and a novel EST was confirmed by semi-quan...Continue Reading

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
May 1, 1992·The Journal of Experimental Medicine·S C BischoffC A Dahinden
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D E HarrisonC Lerner
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S Gartner, H S Kaplan
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·L Sachs
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A L PetzerC J Eaves
Sep 10, 1996·Human Gene Therapy·V W Van Beusechem, D Valerio
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·P W ZandstraC J Eaves
May 25, 2001·International Journal of Hematology·T Nakahata
Jul 17, 2001·Journal of Hematotherapy & Stem Cell Research·L Douay
Jul 21, 2001·Acta Haematologica·M A SmithJ G Smith
Sep 2, 2003·Cytokine & Growth Factor Reviews·Evemie SchutyserJo Van Damme

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Citations

Nov 2, 2005·Biochemical and Biophysical Research Communications·Jingyu ZhangJianwu Dai

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