PMID: 16629173Apr 25, 2006Paper

Gene expression profiling in wild-type and metallothionein mutant fibroblast cell lines

Biological Research
Angela D ArmendárizMauricio González

Abstract

The role of metallothioneins (MT) in copper homeostasis is of great interest, as it appears to be partially responsible for the regulation of intracellular copper levels during adaptation to extracellular excess of the metal. To further investigate a possible role of MTs in copper metabolism, a genomics approach was utilized to evaluate the role of MT on gene expression. Microarray analysis was used to examine the effects of copper overload in fibroblast cells from normal and MT I and II double knock-out mice (MT-/-). As a first step, we compared genes that were significantly upregulated in wild-type and MT-/- cells exposed to copper. Even though wild-type and mutant cells are undistinguishable in terms of their morphological features and rates of growth, our results show that MT-/- cells do not respond with induction of typical markers of cellular stress under copper excess conditions, as observed in the wild-type cell line, suggesting that the transcription initiation rate or the mRNA stability of stress genes is affected when there is an alteration in the copper store capacity. The functional classification of other up-regulated genes in both cell lines indicates that a large proportion (>80%) belong to two major categories:...Continue Reading

Citations

Jun 25, 2009·Physiological Genomics·Min Ok SongJonathan H Freedman
Jan 21, 2014·Progress in Neurobiology·Ivo F ScheiberRalf Dringen
Jan 11, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Patricia MullerLeo W J Klomp
Sep 10, 2008·The American Journal of Clinical Nutrition·Mauricio GonzálezChris Vulpe
Sep 28, 2020·Environmental Research·Akin CayirTimothy M Barrow

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