Gene expression profiling of naïve sheep genetically resistant and susceptible to gastrointestinal nematodes

BMC Genomics
Orla M KeaneJohn C McEwan

Abstract

Gastrointestinal nematodes constitute a major cause of morbidity and mortality in grazing ruminants. Individual animals or breeds, however, are known to differ in their resistance to infection. Gene expression profiling allows us to examine large numbers of transcripts simultaneously in order to identify those transcripts that contribute to an animal's susceptibility or resistance. With the goal of identifying genes with a differential pattern of expression between sheep genetically resistant and susceptible to gastrointestinal nematodes, a 20,000 spot ovine cDNA microarray was constructed. This array was used to interrogate the expression of 9,238 known genes in duodenum tissue of four resistant and four susceptible female lambs. Naïve animals were used in order to look at genes that were differentially expressed in the absence of infection with gastrointestinal nematodes. Forty one unique known genes were identified that were differentially expressed between the resistant and susceptible animals. Northern blotting of a selection of the genes confirmed differential expression. The differentially expressed genes had a variety of functions, although many genes relating to the stress response and response to stimulus were more hi...Continue Reading

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Datasets Mentioned

BETA
GSE3738

Methods Mentioned

BETA
GTPases
electrophoresis
PCR
AFM

Software Mentioned

BLASTN
EASE
BLAST
ImageQuant
MAST
EASEonline
Expression Analysis Systematic Explorer ( EASE )
MEME
CAP3

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