Gene expression profiling of renal dysfunction in rats with experimental cirrhosis

Journal of Hepatology
Marta López-ParraJoan Clària

Abstract

Renal dysfunction is a frequent complication in advanced cirrhosis. The mechanisms underlying this complication have classically been addressed through conventional methods of study of candidate genes, but never on a genome-wide scale. In this investigation, we used microarrays to monitor global gene expression changes in the kidney of cirrhotic rats. Renal samples were obtained from control and carbon tetrachloride-induced cirrhotic rats. RNA samples were reverse-transcribed into Cy5-labeled cDNA, combined with a Cy3-labeled reference and hybridized to oligonucleotide microarrays. Microarrays were scanned in a Genepix 4000B and data analyzed by Luminator v2.0 software. A total of 620 genes were differentially regulated (354 up and 266 down) in the cirrhotic kidney, accounting for approximately 11% of all analyzed transcripts. Functional grouping of these genes revealed that 47 were related to the category of vascular tone and 85 to transporters/channels. Among these, we identified genes and pathways already associated with renal dysfunction as well as a new subset of genes previously unknown to participate in this complication, including a G protein-coupled receptor that binds apelin, a protein phosphatase (calcineurin B) and ...Continue Reading

References

Mar 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C LópezJ Rodés
Mar 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W JiménezJ Rodés
Jan 1, 1993·Current Opinion in Nephrology and Hypertension·M O'SheaM R Hammerman
Oct 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P AngeliJ Rodés
May 15, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Bosch-MarcéJ Rodés
Aug 29, 1998·Journal of Hepatology·J H HenriksenN J Christensen
Mar 30, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Pérez-RuizW Jiménez
Dec 4, 2002·Nature Reviews. Drug Discovery·Atul Butte
Mar 1, 2003·Acta Physiologica Scandinavica·M J Joyner, N M Dietz
Jun 21, 2003·Deutsche medizinische Wochenschrift·A L Gerbes, V Gülberg
Apr 14, 2004·Current Medicinal Chemistry·Sara Martínez-Martínez, Juan Miguel Redondo
May 20, 2004·Kidney International·Bieke F SchrijversAn S De Vriese
Jul 10, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Larry A SlomowitzKaren A Munger
Mar 23, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Paolo AngeliWladimiro Jiménez
Jun 30, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Daryl T-Y LauStanley M Lemon
Jul 7, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Zobair M YounossiVikas Chandhoke

❮ Previous
Next ❯

Citations

Mar 3, 2007·Pflügers Archiv : European journal of physiology·Carsten KneuerKerstin U Honscha
Jan 10, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Giuliano CiarimboliAlex Sparreboom
Aug 8, 2008·Current Opinion in Organ Transplantation·Gianni Biancofiore, Connie L Davis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.