Gene expression regulation by the Curli activator CsgD protein: modulation of cellulose biosynthesis and control of negative determinants for microbial adhesion

Journal of Bacteriology
Eva BrombacherPaolo Landini

Abstract

Curli fibers, encoded by the csgBAC genes, promote biofilm formation in Escherichia coli and other enterobacteria. Curli production is dependent on the CsgD transcription activator, which also promotes cellulose biosynthesis. In this study, we investigated the effects of CsgD expression from a weak constitutive promoter in the biofilm formation-deficient PHL565 strain of E. coli. We found that despite its function as a transcription activator, the CsgD protein is localized in the cytoplasmic membrane. Constitutive CsgD expression promotes biofilm formation by PHL565 and activates transcription from the csgBAC promoter; however, csgBAC expression remains dependent on temperature and the growth medium. Constitutive expression of the CsgD protein results in altered transcription patterns for at least 24 novel genes, in addition to the previously identified CsgD-dependent genes. The cspA and fecR genes, encoding regulatory proteins responding to cold shock and to iron, respectively, and yoaD, encoding a putative negative regulator of cellulose biosynthesis, were found to be some of the novel CsgD-regulated genes. Consistent with the predicted functional role, increased expression of the yoaD gene negatively affects cell aggregation...Continue Reading

References

Jun 1, 1988·Journal of Bacteriology·U PresslerV Braun
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J C LelongG Stöffler
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H M KeW N Lipscomb
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson
Sep 1, 1993·Microbiological Reviews·C MédigueA Danchin
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·M HammarS Normark
Oct 10, 1998·Molecular Microbiology·L A Pratt, T J Silhavy
Dec 10, 1998·Nucleic Acids Research·P D KarpM Krummenacker
Sep 14, 1999·FEMS Microbiology Letters·C DorelP Lejeune
Nov 5, 1999·Biochimica Et Biophysica Acta·K YamanakaS Inouye
Feb 11, 2000·The Journal of Infectious Diseases·Z BianS Normark
Jul 6, 2000·Bioscience, Biotechnology, and Biochemistry·H KawasakiT Utagawa
Sep 15, 2001·FEMS Microbiology Letters·M Y GalperinE V Koonin
Oct 26, 2002·International Journal of Medical Microbiology : IJMM·Adrian L CooksonMartin J Woodward
Jun 26, 2003·Nucleic Acids Research·Elisabeth GasteigerAmos Bairoch
Jul 17, 2003·Molecular Microbiology·Ulrich GerstelUte Römling
Aug 6, 2003·Journal of Molecular Biology·Claudio O GualerziCynthia L Pon

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Jul 5, 2013·Archives of Microbiology·Muriel B Herrington, Chris Sitaras
Mar 1, 2012·The Journal of Microbiology·Sung-Min KimJungmin Kim
Mar 17, 2009·Nature Reviews. Microbiology·Regine Hengge
Jan 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·William H DePasMatthew R Chapman
Sep 4, 2008·Genes & Development·Christina PesaventoRegine Hengge
Jan 19, 2010·Applied and Environmental Microbiology·Michal Weiss-MuszkatShlomo Sela
Apr 21, 2009·Journal of Bacteriology·Jennifer I HandfordTracy Palmer
Jan 12, 2010·Journal of Bacteriology·Alvin LoEric Reynolds
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Ece Karatan, Paula Watnick
Jul 7, 2009·Annual Review of Microbiology·Renee Elizabeth Sockett
Aug 3, 2010·PloS One·Aaron P WhiteMichael G Surette
Mar 31, 2009·Future Microbiology·Kristina JonasUte Römling
Feb 7, 2014·Antonie van Leeuwenhoek·Zoya SamoilovaGalina Smirnova
Feb 27, 2014·BMC Research Notes·José María Gómez-Gómez, Ricardo Amils
Dec 3, 2014·International Journal of Food Microbiology·María del Rosario Aya CastañedaMaría Cristina Cerquetti
Jan 10, 2012·Journal of Biotechnology·Tatyana L Povolotsky, Regine Hengge
Oct 7, 2015·Trends in Microbiology·Nani Van GervenHan Remaut
Jan 24, 2009·Trends in Microbiology·Russell D Monds, George A O'Toole
Nov 12, 2013·Scientific Reports·Valerie W C Soo, Thomas K Wood
Oct 3, 2006·Molecular Microbiology·Harald WeberRegine Hengge
Jun 16, 2010·Molecular Microbiology·Katherina ZakikhanyUte Römling
Sep 9, 2015·Frontiers in Microbiology·Efstathios GiaourisManuel Simões
Jan 15, 2013·Diagnostic Microbiology and Infectious Disease·Lise CrémetNathalie Caroff

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