Gene Expression Response to Sea Lice in Atlantic Salmon Skin: RNA Sequencing Comparison Between Resistant and Susceptible Animals

Frontiers in Genetics
Diego RobledoRoss D Houston

Abstract

Sea lice are parasitic copepods that cause large economic losses to salmon aquaculture worldwide. Frequent chemotherapeutic treatments are typically required to control this parasite, and alternative measures such as breeding for improved host resistance are desirable. Insight into the host-parasite interaction and mechanisms of host resistance can lead to improvements in selective breeding, and potentially novel treatment targets. In this study, RNA sequencing was used to study the skin transcriptome of Atlantic salmon (Salmo salar) parasitized with sea lice (Caligus rogercresseyi). The overall aims were to compare the transcriptomic profile of skin at louse attachment sites and "healthy" skin, and to assess differences in gene expression response between animals with varying levels of resistance to the parasite. Atlantic salmon pre-smolts were challenged with C. rogercresseyi, growth and lice count measurements were taken for each fish. 21 animals were selected and RNA-Seq was performed on skin from a louse attachment site, and skin distal to attachment sites for each animal. These animals were classified into family-balanced groups according to the traits of resistance (high vs. low lice count), and growth during infestation...Continue Reading

References

Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Sep 12, 2002·Biochimica Et Biophysica Acta·Lucy East, Clare M Isacke
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoh-Ichi SekiMasato Kubo
May 22, 2004·Briefings in Bioinformatics·Amos BairochElisabeth Gasteiger
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melania CapassoIan Spendlove
Jul 18, 2006·Nucleic Acids Research·Jianmin WuLiping Wei
Apr 24, 2007·The Journal of Biological Chemistry·Rachel J M AbbottSusan M Lea
Feb 28, 2009·Journal of Fish Diseases·Mark J Costello
Jun 13, 2009·Nature Reviews. Immunology·Teunis B H Geijtenbeek, Sonja I Gringhuis
Sep 14, 2011·Molecules and Cells·Hee Jin ParkDae-Jin Yun
Jan 6, 2012·Proceedings. Biological Sciences·L Neil FrazerMartin Krkosek
Jan 17, 2012·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Laura M BradenSimon R M Jones
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Jan 15, 2013·Journal of Fish Diseases·O TorrissenD Jackson
Mar 20, 2013·BioMed Research International·Alicia Vázquez-MendozaMiriam Rodriguez-Sosa
Sep 5, 2013·Developmental and Comparative Immunology·Mark D Fast
Dec 18, 2013·Cellular Microbiology·J Claire HovingGordon D Brown
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Sep 25, 2014·Annual Review of Marine Science·Kevin D LaffertySonja M Saksida
Dec 3, 2014·Developmental and Comparative Immunology·Laura M BradenSimon R M Jones
Dec 9, 2014·Frontiers in Genetics·Jørgen OdegårdTheo H E Meuwissen
Dec 17, 2014·Frontiers in Genetics·José Manuel YáñezScott Newman
Feb 3, 2015·Trends in Parasitology·Stian Mørch AaenTor Einar Horsberg
Aug 21, 2015·Journal of the Royal Society, Interface·Karim GharbiMichael Stear
Apr 19, 2016·Nature·Sigbjørn LienWilliam S Davidson
Jul 1, 2016·Genetics, Selection, Evolution : GSE·Hsin-Yuan TsaiRoss D Houston
Sep 23, 2016·G3 : Genes - Genomes - Genetics·Christos PalaiokostasLuca Bargelloni

Methods Mentioned

BETA
RNA-Seq

Related Concepts

Breeding
Gene Expression
Genes
Genome
Lice
Parasites
RNA
Salmon
Treatment Protocols
Up-Regulation (Physiology)

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.