Gene expression signatures of breast cancer stem and progenitor cells do not exhibit features of Warburg metabolism

Stem Cell Research & Therapy
Nicole GordonSuellen J Pommier

Abstract

Cancers are believed to adapt to continual changes in glucose and oxygen availability by relying almost exclusively on glycolytic metabolism for energy (i.e. the Warburg effect). The process by which breast cancers sustain growth in avascular tissue is thought to be mediated via aberrant hypoxia response with ensuing shifts in glycolytic metabolism. Given their role in initiating and perpetuating tumors, we sought to determine whether breast cancer stem and progenitor cells play an instrumental role in this adaptive metabolic response. Breast cancer stem/progenitor cells were isolated from invasive ductal carcinomas, and benign stem cells (SC) were isolated from reduction mammoplasty tissues. Relative expression of 33 genes involved in hypoxia and glucose metabolism was evaluated in flow cytometrically isolated stem and progenitor cell populations. Significance between cohorts and cell populations was determined using Student's 2-tailed t test. While benign stem/progenitor cells exhibited few significant inter-group differences in expression of genes involved in hypoxia regulation or glucose metabolism, breast cancer stem/progenitor cells demonstrated significant inter-group variability. Breast cancer stem/progenitor cells adap...Continue Reading

References

Feb 15, 2000·Methods : a Companion to Methods in Enzymology·J E TroskoM Wade
Apr 12, 2002·The Journal of Biological Chemistry·Huasheng LuAjay Verma
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Feb 24, 1956·Science·O WARBURG
Dec 31, 2003·Biochemical and Biophysical Research Communications·Xin Lin Zu, Michael Guppy
Jun 3, 2004·Cancer Research·Rebecca L ElstromCraig B Thompson
Nov 2, 2004·Nature Reviews. Cancer·Robert A Gatenby, Robert J Gillies
Jan 6, 2006·Nature·John StinglConnie J Eaves
Nov 3, 2006·Cancer Research·Martin Götte, George W Yip
Apr 11, 2007·The Journal of Cell Biology·René VilladsenOle W Petersen
Nov 15, 2008·The Journal of Pathology·J Stingl
Oct 20, 2009·Annals of Surgical Oncology·SuEllen J PommierRodney F Pommier
Jul 13, 2011·Annals of Surgical Oncology·Suellen J PommierRodney F Pommier
Oct 6, 2011·Molecular Cancer Research : MCR·Jeanne M V Louderbough, Joyce A Schroeder
Oct 12, 2011·Annual Review of Cell and Developmental Biology·Sophia Y Lunt, Matthew G Vander Heiden
Oct 17, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mayumi TamadaHideyuki Saya
Feb 5, 2013·Antioxidants & Redox Signaling·Rafal R NazarewiczSergey I Dikalov
May 30, 2013·Development·Ng Shyh-ChangLewis C Cantley
Jul 26, 2013·JAMA Surgery·Cory A DonovanSuellen J Pommier
Sep 4, 2013·The Journal of Clinical Investigation·Gregg L Semenza
Sep 26, 2013·Nature Reviews. Cancer·Benjamin Beck, Cédric Blanpain
Jul 11, 2014·Breast Cancer Research and Treatment·Erina VlashiFrank Pajonk
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Dec 3, 2014·Cancer Letters·Jeong Su ParkYoung Yang

❮ Previous
Next ❯

Citations

May 26, 2016·Breast Cancer Research : BCR·Maria Peiris-PagèsMichael P Lisanti
Oct 17, 2016·EBioMedicine·Brett A MorrisPatricia J Keely
Feb 28, 2020·Experimental Biology and Medicine·Yao-An ShenYau-Huei Wei
Dec 20, 2017·International Journal of Molecular Sciences·Jorge S Burns, Gina Manda
Sep 16, 2021·Journal of Neuropathology and Experimental Neurology·Joseph ChenHermann B Frieboes

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence-activated cell sorting
FACS

Software Mentioned

Expression Suite
CellQuest
Sequenome Mass ARRAY

Related Concepts

Related Feeds

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Cancer Metabolic Reprogramming (Keystone)

Cancer metabolic reprogramming is important for the rapid growth and proliferation of cancer cells. Cancer cells have the ability to change their metabolic demands depending on their environment, regulated by the activation of oncogenes or loss of tumor suppressor genes. Here is the latest research on cancer metabolic reprogramming.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Cancer Metabolic Reprogramming

Cancer metabolic reprogramming is important for the rapid growth and proliferation of cancer cells. Cancer cells have the ability to change their metabolic demands depending on their environment, regulated by the activation of oncogenes or loss of tumor suppressor genes. Here is the latest research on cancer metabolic reprogramming.