Gene expression to mitochondrial metabolism: Variability among cultured Trypanosoma cruzi strains

PloS One
Murat C KalemSara L Zimmer

Abstract

The insect-transmitted protozoan parasite Trypanosoma cruzi experiences changes in nutrient availability and rate of flux through different metabolic pathways across its life cycle. The species encompasses much genetic diversity of both the nuclear and mitochondrial genomes among isolated strains. The genetic or expression variation of both genomes are likely to impact metabolic responses to environmental stimuli, and even steady state metabolic function, among strains. To begin formal characterization these differences, we compared aspects of metabolism between genetically similar strains CL Brener and Tulahuen with less similar Esmeraldo and Sylvio X10 strains in a culture environment. Epimastigotes of all strains took up glucose at similar rates. However, the degree of medium acidification that could be observed when glucose was absent from the medium varied by strain, indicating potential differences in excreted metabolic byproducts. Our main focus was differences related to electron transport chain function. We observed differences in ATP-coupled respiration and maximal respiratory capacity, mitochondrial membrane potential, and mitochondrial morphology between strains, despite the fact that abundances of two nuclear-encod...Continue Reading

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Citations

Jan 22, 2020·Molecular Microbiology·Roger Ramirez-BarriosSara L Zimmer
May 29, 2020·PeerJ·Lissa Cruz-SaavedraJuan David Ramírez

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Methods Mentioned

BETA
electron microscopy
RNA-seq

Software Mentioned

blast package
blastp
BEDTools
Studio
bwa
EdgeR
FlowJo
DEseq2
Wave Desktop
Trimmomatic

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