Gene gun bombardment with DNA-coated golden particles enhanced the protective effect of a DNA vaccine based on thioredoxin glutathione reductase of Schistosoma japonicum

BioMed Research International
Yan CaoZhiqiang Fu

Abstract

Schistosomiasis, caused by infection with Schistosoma species, remains an important parasitic zoonosis. Thioredoxin glutathione reductase of Schistosoma japonicum (SjTGR) plays an important role in the development of the parasite and for its survival. Here we present a recombinant plasmid DNA vaccine, pVAX1/SjTGR, to estimate its protection against S. japonicum in BALB/c mice. The DNA vaccine administrated by particle bombardment induced higher protection than by intramuscular injection. All animals vaccinated with pVAX1/SjTGR developed significant specific anti-SjTGR antibodies than control groups. Moreover, animals immunized by gene gun exhibited a splenocyte proliferative response, with an increase in IFN- γ and IL-4. The recombinant plasmid administrated by gene gun achieved a medium protective efficacy of 27.83-38.83% (P < 0.01) of worm reduction and 40.38-44.51% (P < 0.01) of liver egg count reduction. It suggests that different modes of administering a DNA vaccine can influence the protective efficacy induced by the vaccine. Interestingly, from the enzymatic activity results, we found that worms obtained from pVAX1/SjTGR-vaccinated animals expressed lower enzymatic activity than the control group and the antibodies weake...Continue Reading

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Methods Mentioned

BETA
Enzyme-Linked Immunosorbent Assay
FACS
PMA
flow cytometry
Assay
PCR
transfection
ELISA
gene gun

Related Concepts

Thioredoxin glutathione reductase
Helminth Antibodies
Antigens, Helminth
DNA, Double-Stranded
Enzyme-Linked Immunosorbent Assay
Interferon Type II
Mice, Inbred BALB C
Multienzyme Complexes
NADH, NADPH Oxidoreductases
Episomes

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Anthelmintics

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