PMID: 7543587Sep 1, 1995Paper

Gene I mutants of peanut chlorotic streak virus, a caulimovirus, replicate in plants but do not move from cell to cell

Journal of Virology
D A DucasseR J Shepherd

Abstract

Gene I of peanut chlorotic streak virus (PCISV), a caulimovirus, is homologous to gene I of other caulimoviruses and may encode a protein for virus movement. To evaluate the function of gene I, several mutations were created in this gene of an infectious, partially redundant clone of PCISV. Constructs with an in-frame deletion and a single amino acid substitution in gene I were not infectious. To test for replication of these mutants in primarily infected cells, an immunosorbent PCR technique was devised. Virus particles formed by mutants in plants were recovered by binding to antivirus antibodies on a solid matrix and DNase treated to discriminate against residual inoculum, and DNA of trapped virions was subjected to PCR amplification. Gene I mutants were shown to direct formation of encapsidated DNA as revealed by a PCR product. Control gene V mutants (reverse transcriptase essential for replication) did not yield a PCR product. Quantitative PCR allowed estimation of the proportion of cells initially infected by gene I mutants and the amount of extractable virus per cell. It is concluded that PCISV gene I encodes a movement protein and that the immunoselection-PCR technique is useful in studying subliminal virus infection in ...Continue Reading

References

Oct 8, 1992·Nature·P D Siebert, J W Larrick
Apr 17, 1992·Cell·C M DeomR N Beachy
Sep 1, 1991·Journal of Virological Methods·M StiegerJ Mous
Jan 1, 1990·Advances in Virus Research·J G Atabekov, M E Taliansky
May 1, 1990·The Journal of General Virology·U Melcher
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·V CitovskyP Zambryski
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N GrimsleyR Walden

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