Gene induction by interferons: functional complementation between trans-acting factors induced by alpha interferon and gamma interferon.

Molecular and Cellular Biology
S K BandyopadhyayG C Sen

Abstract

HeLaM is a variant cell line in which the transcriptional induction of many genes by alpha interferon has special characteristics (Tiwari et al., Mol. Cell. Biol. 8:4289-4294, 1988). The same characteristics were also displayed for induced transcription of a permanently transfected chimeric gene containing the interferon-stimulated response element of gene 561. For understanding the molecular basis of the special requirements of HeLaM cells, an analysis of the interferon-stimulated gene factors (ISGF) was undertaken. By using gel shift assays, it was shown that the activation of ISGF3 by alpha interferon treatment of HeLaM cells had characteristics identical to those of induced transcription: inhibition by 2-aminopurine and the need for ongoing protein synthesis which was obviated by pretreating the cells with gamma interferon. Upon mixing in vitro the cytoplasmic fraction of gamma interferon-treated HeLaM cells with that of cells treated with alpha interferon and cycloheximide, active ISGF3 was reconstituted, presumably through complementation of two components, ISGF3 gamma and ISGF3 alpha, present in the two respective fractions. Because, unlike other cells, untreated HeLaM cells did not contain detectable levels of either co...Continue Reading

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Citations

Jan 1, 1996·Cancer Investigation·D V Kalvakolanu, E C Borden
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sanjit K RoyDhananjaya V Kalvakolanu
Aug 15, 1991·European Journal of Biochemistry·B R Williams
Dec 27, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Dhananjaya V Kalvakolanu, Sanjit K Roy
Sep 16, 2009·Journal of Immunotherapy·Takaya TsunoKathryn C Zoon
Jan 1, 1995·Cancer Investigation·M L JaramilloJ C Bell
Jan 2, 1993·International Journal of Cancer. Journal International Du Cancer·A S MistchenkoJ Wietzerbin
Jun 7, 2002·The Journal of Biological Chemistry·Junbo HuDhananjaya V Kalvakolanu
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·X WeihuaD V Kalvakolanu
Apr 25, 2000·The Journal of Biological Chemistry·S K RoyD V Kalvakolanu
Jun 1, 1996·Cytokine & Growth Factor Reviews·A R BluyssenD E Levy
Oct 11, 2003·Pharmacology & Therapeutics·Dhananjaya V Kalvakolanu

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