Gene mdpC plays a regulatory role in the methyl-tert-butyl ether degradation pathway of Methylibium petroleiphilum strain PM1

FEMS Microbiology Letters
Geetika JoshiKrassimira R Hristova

Abstract

Among the few bacteria known to utilize methyl tert-butyl ether (MTBE) as a sole carbon source, Methylibium petroleiphilum PM1 is a well-characterized organism with a sequenced genome; however, knowledge of the genetic regulation of its MTBE degradation pathway is limited. We investigated the role of a putative transcriptional activator gene, mdpC, in the induction of MTBE-degradation genes mdpA (encoding MTBE monooxygenase) and mdpJ (encoding tert-butyl alcohol hydroxylase) of strain PM1 in a gene-knockout mutant mdpC(-). We also utilized quantitative reverse transcriptase PCR assays targeting genes mdpA, mdpJ and mdpC to determine the effects of the mutation on transcription of these genes. Our results indicate that gene mdpC is involved in the induction of both mdpA and mdpJ in response to MTBE and tert-butyl alcohol (TBA) exposure in PM1. An additional independent mechanism may be involved in the induction of mdpJ in the presence of TBA.

References

Nov 5, 1999·Applied and Environmental Microbiology·J R HansonK M Scow
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·O Danot
Nov 28, 2001·Applied and Environmental Microbiology·P B HatzingerR J Steffan
Feb 6, 2002·Environmental Science & Technology·Ryan D WilsonKate M Scow
Jun 1, 2002·Applied and Environmental Microbiology·Alan FrançoisFrédéric Monot
Feb 12, 2004·Environmental Microbiology·Akihiro HaraShigeaki Harayama
Jun 15, 2005·Analytical Chemistry·Susan D Richardson, Thomas A Ternes
Jul 20, 2005·Applied Microbiology and Biotechnology·Nicolas Lopes FerreiraCharles W Greer
Apr 22, 2006·International Journal of Systematic and Evolutionary Microbiology·Cindy H NakatsuYoichi Kamagata
Dec 6, 2006·Applied Microbiology and Biotechnology·G M ZaitsevM M Häggblom
Mar 24, 2007·Bioinformatics·Triinu Koressaar, Maido Remm
Apr 7, 2007·Environmental Science & Technology·Mònica RosellHans Hermann Richnow
Jun 7, 2007·International Journal of Systematic and Evolutionary Microbiology·Ute LechnerThore Rohwerder
Dec 18, 2007·Environmental Microbiology·Oleg N RevaDavid W Ussery
Sep 16, 2008·Applied and Environmental Microbiology·Radomir SchmidtKrassimira R Hristova
May 25, 2012·Water Research·Katharine P NorthKate M Scow
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Jan 30, 2013·Ground Water Monitoring & Remediation·Katharine P NorthKate M Scow
Jul 1, 1985·Evolution; International Journal of Organic Evolution·Joseph Felsenstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.