Gene network and pathway analysis of bovine mammary tissue challenged with Streptococcus uberis reveals induction of cell proliferation and inhibition of PPARgamma signaling as potential mechanism for the negative relationships between immune response and lipid metabolism.

BMC Genomics
Kasey M MoyesJuan J Loor

Abstract

Information generated via microarrays might uncover interactions between the mammary gland and Streptococcus uberis (S. uberis) that could help identify control measures for the prevention and spread of S. uberis mastitis, as well as improve overall animal health and welfare, and decrease economic losses to dairy farmers. The main objective of this study was to determine the most affected gene networks and pathways in mammary tissue in response to an intramammary infection (IMI) with S. uberis and relate these with other physiological measurements associated with immune and/or metabolic responses to mastitis challenge with S. uberis O140J. Streptococcus uberis IMI resulted in 2,102 (1,939 annotated) differentially expressed genes (DEG). Within this set of DEG, we uncovered 20 significantly enriched canonical pathways (with 20 to 61 genes each), the majority of which were signaling pathways. Among the most inhibited were LXR/RXR Signaling and PPARalpha/RXRalpha Signaling. Pathways activated by IMI were IL-10 Signaling and IL-6 Signaling which likely reflected counter mechanisms of mammary tissue to respond to infection. Of the 2,102 DEG, 1,082 were up-regulated during IMI and were primarily involved with the immune response, e.g...Continue Reading

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Citations

Oct 5, 2011·Journal of Mammary Gland Biology and Neoplasia·Juan J LoorMassimo Bionaz
Oct 20, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sandra HalwachsWalther Honscha
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Nov 3, 2010·Veterinary Immunology and Immunopathology·Manuela RinaldiAnthony V Capuco
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Sep 25, 2016·Research in Veterinary Science·Xing ChenShujun Zhang
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Aug 28, 2019·The Journal of Dairy Research·Mayara M D C A WellerMarta F Martins
Jan 1, 2014·Journal of Veterinary Medicine·Mohamed Ezzat AlnakipJorge Barros-Velázquez

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Methods Mentioned

BETA
biopsies
reverse transcription-PCR
biopsy
PCR

Software Mentioned

IPA
GeneSpring GX7
GO
Ingenuity Pathway Analysis
GenePix
GeneSpring GX
BLASTN
Excel
Ingenuity Pathways Analysis ( IPA )
IPA Base

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