Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Environmental Microbiology
Evangelos MourkasSamuel K Sheppard

Abstract

The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food-borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here, we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. AMR was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug-resistant genomic islands (GIs), often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among ...Continue Reading

References

Dec 1, 1988·Antimicrobial Agents and Chemotherapy·R ZilhaoP Courvalin
Dec 1, 1983·Antimicrobial Agents and Chemotherapy·D E TaylorB J Allan
May 5, 2000·International Journal of Antimicrobial Agents·A E van den Bogaard, E E Stobberingh
Jan 4, 2001·Journal of Clinical Microbiology·K E DingleM C Maiden
Apr 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B M Allos
Jun 9, 2001·International Journal of Antimicrobial Agents·S SchwarzT R Walsh
Oct 6, 2001·Current Opinion in Microbiology·M Teuber
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Nov 25, 2003·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Guido WernerWolfgang Witte
Dec 9, 2003·Applied and Environmental Microbiology·F M CollesM C J Maiden
Jul 17, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Amera GibreelDiane E Taylor
May 27, 2005·Antimicrobial Agents and Chemotherapy·David A Alfredson, Victoria Korolik
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Adeline AchardRoland Leclercq
May 23, 2006·Microbes and Infection·Sophie PayotQijing Zhang
Jun 1, 2006·The Journal of Antimicrobial Chemotherapy·Amera Gibreel, Diane E Taylor
Aug 4, 2006·Applied and Environmental Microbiology·Siddhartha ThakurWondwossen A Gebreyes
Aug 3, 2007·International Journal of Antimicrobial Agents·David M Livermore
Apr 12, 2008·Science·Samuel K SheppardMartin C J Maiden
Nov 15, 2008·Molecular Biology and Evolution·Daniel J WilsonPaul Fearnhead
Dec 24, 2008·Animal Health Research Reviews·Patrick Boerlin, Richard J Reid-Smith
Mar 5, 2009·Future Microbiology·Taradon LuangtongkumQijing Zhang
Mar 10, 2009·International Journal of Food Microbiology·Samuel K SheppardKen J Forbes
Mar 12, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Samuel K SheppardKen J Forbes
Oct 21, 2009·Foodborne Pathogens and Disease·Iain D OgdenNorval J C Strachan
Jun 8, 2010·Applied and Environmental Microbiology·Samuel K SheppardNoel D McCarthy
Dec 15, 2010·BMC Bioinformatics·Keith A Jolley, Martin C J Maiden
Dec 21, 2010·Bioinformatics·Klaus Peter Schliep
Jul 26, 2011·Applied and Environmental Microbiology·Emma L SprostonNorval J C Strachan
Dec 3, 2011·Vector Borne and Zoonotic Diseases·David HermansFreddy Haesebrouck
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Nov 7, 2012·Antimicrobial Agents and Chemotherapy·Marta TothSergei B Vakulenko
Jan 3, 2013·Molecular Ecology·Samuel K SheppardDaniel Falush

❮ Previous
Next ❯

Citations

Nov 7, 2019·Ecology and Evolution·Arthur EscalasJizhong Zhou
May 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Evangelos MourkasSamuel K Sheppard
Jun 3, 2020·Microbial Biotechnology·Zulema Udaondo, María José Huertas
Nov 13, 2020·Frontiers in Cellular and Infection Microbiology·Francesca MarottaGiuliano Garofolo
Feb 5, 2021·Nature Communications·Leonardos MageirosSamuel K Sheppard
Nov 5, 2020·Microorganisms·Lorena HormeñoAlberto Quesada
Jul 7, 2021·Expert Review of Anti-infective Therapy·Giorgia SulisSumanth Gandra
May 16, 2021·Applied and Environmental Microbiology·Luyao MaXiaonan Lu
Aug 17, 2021·BioMed Research International·Md Ashikur RahmanS M Lutful Kabir
Aug 10, 2021·Antimicrobial Agents and Chemotherapy·Quentin JehannePhilippe Lehours

❮ Previous
Next ❯

Datasets Mentioned

BETA
CP023546.1
CP017030.1
CP002030.1
MG812141.1

Methods Mentioned

BETA
PCR

Software Mentioned

MLST
Stata
SnapGene
R
phangorn
R Phangorn
BIGSdb
SPAdes
ResFinder
MAFFT

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.