Gene presence-absence variation associates with quantitative Verticillium longisporum disease resistance in Brassica napus.

Scientific Reports
Iulian GaburChristian Obermeier

Abstract

Although copy number variation (CNV) and presence-absence variation (PAV) have been discovered in selected gene families in most crop species, the global prevalence of these polymorphisms in most complex genomes is still unclear and their influence on quantitatively inherited agronomic traits is still largely unknown. Here we analyze the association of gene PAV with resistance of oilseed rape (Brassica napus) against the important fungal pathogen Verticillium longisporum, as an example for a complex, quantitative disease resistance in the strongly rearranged genome of a recent allopolyploid crop species. Using Single Nucleotide absence Polymorphism (SNaP) markers to efficiently trace PAV in breeding populations, we significantly increased the resolution of loci influencing V. longisporum resistance in biparental and multi-parental mapping populations. Gene PAV, assayed by resequencing mapping parents, was observed in 23-51% of the genes within confidence intervals of quantitative trait loci (QTL) for V. longisporum resistance, and high-priority candidate genes identified within QTL were all affected by PAV. The results demonstrate the prominent role of gene PAV in determining agronomic traits, suggesting that this important cla...Continue Reading

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Citations

Aug 2, 2020·Plant Biotechnology Journal·Harmeet Singh ChawlaRod J Snowdon
Jan 21, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Paul VollrathChristian Obermeier
Feb 2, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Dominic KnochThomas Altmann
Feb 10, 2021·Genes·Matteo MartinaArnaud G Bovy
Mar 9, 2021·Frontiers in Genetics·Nur Shuhadah Mohd SaadJacqueline Batley
Jun 10, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junrey AmasJacqueline Batley
May 26, 2021·Plant Biotechnology Journal·Dandan HuJun Zou
Jul 22, 2021·The Plant Genome·Philipp E BayerDavid Edwards

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Methods Mentioned

BETA
genotyping
dissection
Illumina sequencing
chip
PCR
RNASeq
RNA-Seq

Software Mentioned

runCharacterize
BLASTn
IrysSolve
bedtools
SGSGeneLoss
CLC Genomics Workbench
R package GenABEL
Irys
MSTMap
Knickers

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