Gene profile of zebrafish fin regeneration offers clues to kinetics, organization and biomechanics of basement membrane

Matrix Biology : Journal of the International Society for Matrix Biology
Pauline NauroyFlorence Ruggiero

Abstract

How some animals regenerate missing body parts is not well understood. Taking advantage of the zebrafish caudal fin model, we performed a global unbiased time-course transcriptomic analysis of fin regeneration. Biostatistics analyses identified extracellular matrix (ECM) as the most enriched gene sets. Basement membranes (BMs) are specialized ECM structures that provide tissues with structural cohesion and serve as a major extracellular signaling platform. While the embryonic formation of BM has been extensively investigated, its regeneration in adults remains poorly studied. We therefore focused on BM gene expression kinetics and showed that it recapitulates many aspects of development. As such, the re-expression of the embryonic col14a1a gene indicated that col14a1a is part of the regeneration-specific program. We showed that laminins and col14a1a genes display similar kinetics and that the corresponding proteins are spatially and temporally controlled during regeneration. Analysis of our CRISPR/Cas9-mediated col14a1a knockout fish showed that collagen XIV-A contributes to timely deposition of laminins. As changes in ECM organization can affect tissue mechanical properties, we analyzed the biomechanics of col14a1a-/- regenera...Continue Reading

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Citations

Sep 22, 2019·Development·Ines J MarquesNadia Mercader
Oct 23, 2019·Frontiers in Endocrinology·Johannes R SchmidtFranziska Knopf
Jul 16, 2020·Scientific Reports·Thomas BiseAnna Jaźwińska
Jan 31, 2021·Cells·Valentina DaponteAntonella Forlino

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