Gene profiling of bone around orthodontic mini-implants by RNA-sequencing analysis

BioMed Research International
Kyung-Yen NahmSeong-Hun Kim

Abstract

This study aimed to evaluate the genes that were expressed in the healing bones around SLA-treated titanium orthodontic mini-implants in a beagle at early (1-week) and late (4-week) stages with RNA-sequencing (RNA-Seq). Samples from sites of surgical defects were used as controls. Total RNA was extracted from the tissue around the implants, and an RNA-Seq analysis was performed with Illumina TruSeq. In the 1-week group, genes in the gene ontology (GO) categories of cell growth and the extracellular matrix (ECM) were upregulated, while genes in the categories of the oxidation-reduction process, intermediate filaments, and structural molecule activity were downregulated. In the 4-week group, the genes upregulated included ECM binding, stem cell fate specification, and intramembranous ossification, while genes in the oxidation-reduction process category were downregulated. GO analysis revealed an upregulation of genes that were related to significant mechanisms, including those with roles in cell proliferation, the ECM, growth factors, and osteogenic-related pathways, which are associated with bone formation. From these results, implant-induced bone formation progressed considerably during the times examined in this study. The upr...Continue Reading

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Citations

Aug 27, 2021·Molecular Cancer Therapeutics·Paul RossmanDeborah W Knapp

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Methods Mentioned

BETA
scanning
RNA-Seq

Software Mentioned

Sequence Detector
Ensembl

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