Gene redundancy and gene compensation: An updated view

Journal of Genetics and Genomics = Yi Chuan Xue Bao
Jinrong Peng

Abstract

Gene knockdown approaches using antisense oligo nucleotides or analogs such as siRNAs and morpholinos have been widely adopted to study gene functions although the off-target issue has been always a concern in these studies. On the other hand, classic genetic analysis relies on the availability of loss-of-function or gain-of-function mutants. The fast development of genome editing technologies such as TALEN and CRISPR/Cas9 has greatly facilitated the generation of null mutants for the functional studies of target genes in a variety of organisms such as zebrafish. Surprisingly, an unexpected discrepancy was observed between morphant phenotype and mutant phenotype for many genes in zebrafish, i.e., while the morphant often displays an obvious phenotype, the corresponding null mutant appears relatively normal or only exhibits a mild phenotype due to gene compensation. Two recent reports have partially answered this intriguing question by showing that a pre-mature termination codon and homologous sequence are required to elicit the gene compensation and the histone modifying complex COMPASS is involved in activating the expression of the compensatory genes. Here, I summarize these exciting new progress and try to redefine the conce...Continue Reading

Citations

Sep 16, 2020·Current Protocols in Immunology·Ben F BrianTanya S Freedman
Dec 7, 2019·Archives of Sexual Behavior·Jackson CliveVincent Savolainen
Jan 6, 2021·International Journal of Molecular Sciences·Giancarlo IarossiMatteo Bertelli
Nov 21, 2020·Scientific Reports·Revathi RamanPudur Jagadeeswaran
Feb 26, 2021·Biochemical and Biophysical Research Communications·Zongzhuang WenJiangang Gao
Mar 9, 2021·Developmental Biology·Anirban BardhanCharles A Ettensohn
Apr 1, 2021·Disease Models & Mechanisms·Abdul Jalil RufaihahCitra N Z Mattar
Oct 20, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hua TengLingqian Wu

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