Gene regulation of Sclerotinia sclerotiorum during infection of Glycine max: on the road to pathogenesis

BMC Genomics
Nathaniel M WestrickMehdi Kabbage

Abstract

Sclerotinia sclerotiorum is a broad-host range necrotrophic pathogen which is the causative agent of Sclerotinia stem rot (SSR), and a major disease of soybean (Glycine max). A time course transcriptomic analysis was performed in both compatible and incompatible soybean lines to identify pathogenicity and developmental factors utilized by S. sclerotiorum to achieve pathogenic success. A comparison of genes expressed during early infection identified the potential importance of toxin efflux and nitrogen metabolism during the early stages of disease establishment. The later stages of infection were characterized by an apparent shift to survival structure formation. Analysis of genes highly upregulated in-planta revealed a temporal regulation of hydrolytic and detoxification enzymes, putative secreted effectors, and secondary metabolite synthesis genes. Redox regulation also appears to play a key role during the course of infection, as suggested by the high expression of genes involved in reactive oxygen species production and scavenging. Finally, distinct differences in early gene expression were noted based on the comparison of S. sclerotiorum infection of resistant and susceptible soybean lines. Although many potential virulenc...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 13, 1998·Molecular Plant-microbe Interactions : MPMI·A ten HaveJ A van Kan
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 9, 2002·FEMS Microbiology Letters·Pascale CottonMichel Fevre
Sep 16, 2003·Molecular Plant-microbe Interactions : MPMI·Jeffrey A Rollins
Jan 28, 2005·Journal of Chemical Ecology·M Soledade C Pedras, Pearson W K Ahiahonu
Apr 9, 2005·Revista iberoamericana de micología·Aitor RementeriaJavier Garaizar
Jul 5, 2005·Journal of Lipid Research·Caiqing Mo, Martin Bard
May 23, 2006·Current Opinion in Plant Biology·Barbara J Howlett
Mar 24, 2007·Bioinformatics·Triinu Koressaar, Maido Remm
Jun 15, 2007·Fungal Genetics and Biology : FG & B·Daigo TakemotoBarry Scott
Oct 12, 2007·Bioscience, Biotechnology, and Biochemistry·Emi SakunoHiromitsu Nakajima
Apr 9, 2008·Molecular Plant-microbe Interactions : MPMI·Kyoung Su KimMartin B Dickman
Jan 22, 2009·The Plant Journal : for Cell and Molecular Biology·Francesca L StefanatoHenk-jan Schoonbeek
Dec 24, 2009·Medical Mycology·Bruno Aluisio Coutinho de Assis TaccoCélia Maria de Almeida Soares
Jan 16, 2010·Nature·Jeremy SchmutzScott A Jackson
Jan 19, 2010·Molecular Plant Pathology·Zafer Dallal BashiMohammed H Borhan
Mar 4, 2010·Genome Biology·Mark D Robinson, Alicia Oshlack
Apr 23, 2010·Journal of Proteome Research·Yue LiangNat N V Kav
Jan 1, 2006·Molecular Plant Pathology·Melvin D BoltonBerlin D Nelson
Sep 6, 2011·Applied and Environmental Microbiology·Hyo-jin KimMartin B Dickman
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Nov 6, 2012·Current Opinion in Microbiology·Paul TudzynskiUlrike Siegmund
Dec 21, 2012·Molecular Plant-microbe Interactions : MPMI·Liangsheng Xu, Weidong Chen

❮ Previous
Next ❯

Citations

Oct 25, 2020·Trends in Plant Science·Guru JagadeeswaranAndrew J Mort
Mar 16, 2021·Natural Product Research·Larissa L S LaraCristiane M Cazal
May 18, 2021·Frontiers in Microbiology·Sivasubramanian Rajarammohan
May 18, 2021·Frontiers in Plant Science·Nathaniel M WestrickMehdi Kabbage

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA Seq
RNA-Seq
RNAseq
PCR
reverse

Software Mentioned

Blast2GO
BlastGo
Primer3
BlastP
Primer
Protein BLAST
FunCat
R Limma package
Subjunc
edgeR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.