PMID: 16643725Apr 29, 2006Paper

Gene regulatory mechanism of hairy and enhancer of split related-1 for angiogenesis factors

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology
Bin LiJing Meng

Abstract

To study the roles of hairy and enhancer of split related-1 (HESR-1) in maintenance of the mature, quiescent vessel and angiogenesis. Human umbilical vein endothelial cells (HUVEC) were cultured. The full-length coding sequence of HESR-1 was cloned into PcDNA3.1 + using standard protocols. HESR-1 specific siRNA was synthesized and cloned into the RNAi-Ready pSIREN-RetroQ ZsGreen Vector. The constructed PcDNA3.1 + HESR-1 plasmid were transfected into HUVEC for the overexpression of HESR-1, and HESR-1-RNAi plasmid were transfected into HUVEC to silence the HESR-1 gene, the expression of KDR, ALK-1 and Ang-1 in HUVEC were analyzed by RT-PCR and Western blot. The expression of KDR was down-regulated and ALK-1 and Ang-1 were up-regulated in HUVEC with the overexpression of HESR-1; The expression of KDR was up-regulated and ALK-1 and Ang-1 were down-regulated the HESR-1 in HUVEC by RNAi. HESR-1 may play an important role in maintenance of vessel in quiescent and control angiogenesis by the regulation of the expression of KDR, ALK-1 and Ang-1.

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