PMID: 9642182Jun 27, 1998Paper

Gene replacement analysis of the Streptomyces virginiae barA gene encoding the butyrolactone autoregulator receptor reveals that BarA acts as a repressor in virginiamycin biosynthesis

Journal of Bacteriology
H NakanoYasuhiro Yamada

Abstract

Virginiae butanolides (VBs), which are among the butyrolactone autoregulators of Streptomyces species, act as a primary signal in Streptomyces virginiae to trigger virginiamycin biosynthesis and possess a specific binding protein, BarA. To clarify the in vivo function of BarA in the VB-mediated signal pathway that leads to virginiamycin biosynthesis, two barA mutant strains (strains NH1 and NH2) were created by homologous recombination. In strain NH1, an internal 99-bp EcoT14I fragment of barA was deleted, resulting in an in-frame deletion of 33 amino acid residues, including the second helix of the probable helix-turn-helix DNA-binding motif. With the same growth rate as wild-type S. virginiae on both solid and liquid media, strain NH1 showed no apparent changes in its morphological behavior, indicating that the VB-BarA pathway does not participate in morphological control in S. virginiae. In contrast, virginiamycin production started 6 h earlier in strain NH1 than in the wild-type strain, demonstrating for the first time that BarA is actively engaged in the control of virginiamycin production and implying that BarA acts as a repressor in virginiamycin biosynthesis. In strain NH2, an internal EcoNI-SmaI fragment of barA was re...Continue Reading

References

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Citations

Jul 28, 2011·Applied Microbiology and Biotechnology·Zahra Salehi-NajafabadiJuan F Martín
Jan 17, 2012·Applied Microbiology and Biotechnology·Yung-Hun YangByung-Gee Kim
Oct 20, 2014·Applied Microbiology and Biotechnology·Zhen-Xing ZhouYong-Quan Li
Feb 9, 2017·Acta Crystallographica. Section F, Structural Biology Communications·Nurhikmah Mohd-SharifChyan Leong Ng
Jan 4, 2020·World Journal of Microbiology & Biotechnology·Haiyang XiaYong-Quan Li
Feb 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jun-ya KatoSueharu Horinouchi
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Juan L RamosRaquel Tobes
Feb 8, 2006·Journal of Industrial Microbiology & Biotechnology·Eric Cundliffe
Feb 14, 2002·Chemistry & Biology·George Stratigopoulos, Eric Cundliffe
Nov 26, 2010·Chemical Reviews·Joanne M Willey, Alisa A Gaskell

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