Gene silencing by chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI) is mediated by transcriptional corepressors, nuclear receptor-corepressor (N-CoR) and silencing mediator for retinoic acid receptor and thyroid hormone receptor (SMRT)

Molecular Endocrinology
Hirotaka ShibataM J Tsai

Abstract

Chicken ovalbumin upstream promoter-transcription factors (COUP-TFs) are orphan receptors that belong to the steroid/thyroid hormone receptor (TR) superfamily and can repress the transcriptional activity of several target genes; however, the precise mechanism of this repression is unknown. Transfection of a Gal4 DNA-binding domain fused to the putative ligand-binding domain of COUP-TFI (Gal4-COUP-TFI) significantly represses the basal transcriptional activity of a reporter gene containing Gal4-binding sites. Cotransfection of COUP-TFI can relieve the Gal4-COUP-TFI repression in a dose-dependent manner. In contrast, COUP-TFI delta35, which lacks the repressor domain (the C-terminal 35 amino acids), fails to relieve this repression. This finding suggests that the repressor domain of COUP-TFI may squelch a limiting amount of corepressor in HeLa cells. In addition, increasing concentrations of TRbeta also can relieve the COUP-TFI repression in a hormone-sensitive manner. Similarly, overexpression of increasing concentration of COUP-TFI, but not COUP-TFI delta35, can squelch the silencing activity of the unliganded TRbeta. Collectively, these results indicate that COUP-TFI and TRbeta share a common corepressor(s) for their silencing...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerR M Evans
Oct 1, 1992·Molecular and Cellular Biology·P TranM Pfahl
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·F A PereiraS Y Tsai
Jan 1, 1994·Annual Review of Biochemistry·M J Tsai, B W O'Malley
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F MargolinF J Rauscher
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·R WitzgallJ V Bonventre
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A BaniahmadB W O'Malley
Jun 17, 1993·Nature·S SahaM Ptashne
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z NawazB W O'Malley
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·J D ChenR M Evans
Nov 1, 1996·The Journal of Biological Chemistry·S L MarcusR A Rachubinski
Jun 1, 1996·Trends in Genetics : TIG·W Hanna-Rose, U Hansen
Aug 1, 1994·Trends in Endocrinology and Metabolism : TEM·Y QiuM J Tsai

❮ Previous
Next ❯

Citations

Mar 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Masashi OkamuraJuro Sakai
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·E J ParkJ D Chen
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y D WenE Seto
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C ForsbergE H Bresnick
Jan 23, 1999·The Journal of Biological Chemistry·S H Hong, M L Privalsky
Jan 8, 2010·The Journal of Biological Chemistry·Ayano Murai-TakedaHiroshi Itoh
Jun 24, 1998·Genes & Development·J ZhangM A Lazar
Jan 5, 2000·Genes & Development·V PerissiM G Rosenfeld
Sep 7, 2002·Molecular and Cellular Biology·Anna N MoraitisCatherine C Thompson
Feb 24, 2012·Journal of Lipid Research·Kezhi Dai, M Mahmood Hussain
Sep 12, 2012·Journal of Molecular Endocrinology·Lacey M Litchfield, Carolyn M Klinge
Mar 28, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Ryo Kominami
Mar 10, 2001·Molecular and Cellular Biology·X HuM A Lazar
Apr 18, 2008·The Cerebellum·Grant W Anderson
Jun 9, 2000·Annual Review of Physiology·J Zhang, M A Lazar
Oct 22, 2003·Molecular Genetics and Metabolism·Robert Clipsham, Edward R B McCabe
Oct 26, 2010·Immunological Reviews·Pentao LiuShannon Burke
Jun 1, 2014·Biochimica Et Biophysica Acta·Ke TangMing-Jer Tsai
Apr 2, 2011·Biochimica Et Biophysica Acta·Audrey Poupeau, Catherine Postic
Nov 11, 2015·BMC Cancer·Sujitra DetchokulAlbert G Frauman
Apr 27, 2007·The FEBS Journal·Ji-Eun AhnKeyan Zhu-Salzman
Dec 4, 2003·Molecular Reproduction and Development·Alexandra Zilz, Geoffrey M Cooper
Dec 6, 2017·Thyroid : Official Journal of the American Thyroid Association·Xiaochun TengGregory A Brent
Jan 1, 2015·Journal of Experimental Neuroscience·Ana Maria OlivaresNeena B Haider
Dec 4, 2003·Thyroid : Official Journal of the American Thyroid Association·Grant W AndersonSidney A Jones
Dec 2, 2008·Nuclear Receptor Signaling·Lei Wang, Chih-Cheng Tsai
Jun 16, 1999·Endocrine Reviews·N J McKennaB W O'Malley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.