Polycomb group (PcG) proteins are required for normal differentiation and development and are frequently deregulated in cancer. PcG proteins are involved in gene silencing; however, their role in initiation and maintenance of transcriptional repression is not well defined. Here, we show that knockout of the Polycomb repressive complex 2 (PRC2) does not lead to significant gene expression changes in mouse embryonic stem cells (mESCs) and that it is dispensable for initiating silencing of target genes during differentiation. Transcriptional inhibition in mESCs is sufficient to induce genome-wide ectopic PRC2 recruitment to endogenous PcG target genes found in other tissues. PRC2 binding analysis shows that it is restricted to nucleosome-free CpG islands (CGIs) of untranscribed genes. Our results show that it is the transcriptional state that governs PRC2 binding, and we propose that it binds by default to nontranscribed CGI genes to maintain their silenced state and to protect cell identity.
Gene silencing triggers Polycomb Repressive Complex 2 recruitment to CpG islands genome-wide [RNA-seq_differentiation]
Gene silencing triggers Polycomb Repressive Complex 2 recruitment to CpG islands genome-wide [ChIP-seq_Input]
Gene silencing triggers Polycomb Repressive Complex 2 recruitment to CpG islands genome-wide [ChIP-seq_drugs]
Gene silencing triggers Polycomb Repressive Complex 2 recruitment to CpG islands genome-wide [iTC-seq_drugs]
The histone methyltransferases Trithorax and Ash1 prevent transcriptional silencing by Polycomb group proteins
SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex
EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency
Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2
Metabolic labeling of RNA uncovers principles of RNA production and degradation dynamics in mammalian cells
Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation
An interspecies analysis reveals a key role for unmethylated CpG dinucleotides in vertebrate Polycomb complex recruitment
Polycomb associates genome-wide with a specific RNA polymerase II variant, and regulates metabolic genes in ESCs
In vivo Polycomb kinetics and mitotic chromatin binding distinguish stem cells from differentiated cells
Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put
Imprinting control regions (ICRs) are marked by mono-allelic bivalent chromatin when transcriptionally inactive
Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
The decrease in histone methyltransferase EZH2 in response to fluid shear stress alters endothelial gene expression and promotes quiescence
Reprogramming of Polycomb-Mediated Gene Silencing in Embryonic Stem Cells by the miR-290 Family and the Methyltransferase Ash1l
Distinct temporal changes in host cell lncRNA expression during the course of an adenovirus infection
Primary Cilium-Autophagy-Nrf2 (PAN) Axis Activation Commits Human Embryonic Stem Cells to a Neuroectoderm Fate
CpG island erosion, polycomb occupancy and sequence motif enrichment at bivalent promoters in mammalian embryonic stem cells
The controversial role of the Polycomb group proteins in transcription and cancer: how much do we not understand Polycomb proteins?
Ctbp2 Modulates NuRD-Mediated Deacetylation of H3K27 and Facilitates PRC2-Mediated H3K27me3 in Active Embryonic Stem Cell Genes During Exit from Pluripotency
Nuclear Fractionation Reveals Thousands of Chromatin-Tethered Noncoding RNAs Adjacent to Active Genes
A fine balance: epigenetic control of cellular quiescence by the tumor suppressor PRDM2/RIZ at a bivalent domain in the cyclin a gene
A hyper-dynamic nature of bivalent promoter states underlies coordinated developmental gene expression modules
Enhancer of zeste homolog-2 (EZH2) methyltransferase regulates transgelin/smooth muscle-22α expression in endothelial cells in response to interleukin-1β and transforming growth factor-β2
DNA methylation and histone modifications regulate SOX11 expression in lymphoid and solid cancer cells
Mechanical regulation of transcription controls Polycomb-mediated gene silencing during lineage commitment
Sustained expression of FMR1 mRNA from reactivated fragile X syndrome alleles after treatment with small molecules that prevent trimethylation of H3K27
Reversible Regulation of Promoter and Enhancer Histone Landscape by DNA Methylation in Mouse Embryonic Stem Cells
Impact of combinatorial dysfunctions of Tet2 and Ezh2 on the epigenome in the pathogenesis of myelodysplastic syndrome
Epigenomics-Based Identification of Major Cell Identity Regulators within Heterogeneous Cell Populations
RSV-Induced H3K4 Demethylase KDM5B Leads to Regulation of Dendritic Cell-Derived Innate Cytokines and Exacerbates Pathogenesis In Vivo
A Cbx8-containing polycomb complex facilitates the transition to gene activation during ES cell differentiation
PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes
Systems Level Analysis of Histone H3 Post-translational Modifications (PTMs) Reveals Features of PTM Crosstalk in Chromatin Regulation.
Whole-Transcriptome Sequencing: a Powerful Tool for Vascular Tissue Engineering and Endothelial Mechanobiology
How Polycomb-Mediated Cell Memory Deals With a Changing Environment: Variations in PcG complexes and proteins assortment convey plasticity to epigenetic regulation as a response to environment
MUC1-C activates polycomb repressive complexes and downregulates tumor suppressor genes in human cancer cells
Conserved RNA-binding specificity of polycomb repressive complex 2 is achieved by dispersed amino acid patches in EZH2
DNA binding by PHF1 prolongs PRC2 residence time on chromatin and thereby promotes H3K27 methylation
Variant PRC1 competes with retinoic acid-related signals to repress Meis2 in the mouse distal forelimb bud
Similarities and differences in the regulation of HoxD genes during chick and mouse limb development
Genome-wide analyses reveal a role of Polycomb in promoting hypomethylation of DNA methylation valleys
The Methylcytosine Dioxygenase Ten-Eleven Translocase-2 (tet2) Enables Elevated GnRH Gene Expression and Maintenance of Male Reproductive Function.
Dangerous liaisons: interplay between SWI/SNF, NuRD, and Polycomb in chromatin regulation and cancer
Ready, Set…Poised!: Polycomb target genes are bound by poised RNA polymerase II throughout differentiation
A comparative epigenome analysis of gammaherpesviruses suggests cis-acting sequence features as critical mediators of rapid polycomb recruitment
A novel role of metal response element binding transcription factor 2 at the Hox gene cluster in the regulation of H3K27me3 by polycomb repressive complex 2
An annotated list of bivalent chromatin regions in human ES cells: a new tool for cancer epigenetic research
Curse of the devil: molecular insights into the emergence of transmissible cancers in the Tasmanian devil (Sarcophilus harrisii)
Classifying human promoters by occupancy patterns identifies recurring sequence elements, combinatorial binding, and spatial interactions
Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity
CATACOMB: An endogenous inducible gene that antagonizes H3K27 methylation activity of Polycomb repressive complex 2 via an H3K27M-like mechanism
Parallel PRC2/cPRC1 and vPRC1 pathways silence lineage-specific genes and maintain self-renewal in mouse embryonic stem cells
Dual functionality of cis-regulatory elements as developmental enhancers and Polycomb response elements
PRC2 Is Dispensable in Vivo for β-Catenin-Mediated Repression of Chondrogenesis in the Mouse Embryonic Cranial Mesenchyme
Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells
PTE, a novel module to target Polycomb Repressive Complex 1 to the human cyclin D2 (CCND2 ) oncogene
Preparation of RNA 3' End Sequencing Libraries of Total and 4-thiouracil Labeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae
Impact of genome architecture on the functional activation and repression of Hox regulatory landscapes
suz12 inactivation in p53 - and nf1 -deficient zebrafish accelerates the onset of malignant peripheral nerve sheath tumors and expands the spectrum of tumor types
Polycomb Repressive Complex 2 is essential for development and maintenance of a functional TEC compartment
ncHMR detector: a computational framework to systematically reveal non-classical functions of histone modification regulators
H3K79me2/3 controls enhancer-promoter interactions and activation of the pan-cancer stem cell marker PROM1/CD133 in MLL-AF4 leukemia cells.
Morphine leads to global genome changes in H3K27me3 levels via a Polycomb Repressive Complex 2 (PRC2) self-regulatory mechanism in mESCs
Reevaluating the roles of histone-modifying enzymes and their associated chromatin modifications in transcriptional regulation.
Epigenome-wide meta-analysis of DNA methylation differences in prefrontal cortex implicates the immune processes in Alzheimer's disease.
A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2
Cell Signaling Coordinates Global PRC2 Recruitment and Developmental Gene Expression in Murine Embryonic Stem Cells
Polycomb Gene Silencing Mechanisms: PRC2 Chromatin Targeting, H3K27me3 'Readout', and Phase Separation-Based Compaction.
The persistent organochlorine pesticide endosulfan modulates multiple epigenetic regulators with oncogenic potential in MCF-7 cells
ATRX In-Frame Fusion Neuroblastoma Is Sensitive to EZH2 Inhibition via Modulation of Neuronal Gene Signatures
Widespread reorganisation of pluripotent factor binding and gene regulatory interactions between human pluripotent states.
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