Gene stacking by recombinases

Plant Biotechnology Journal
Vibha Srivastava, James Thomson

Abstract

Efficient methods of stacking genes into plant genomes are needed to expedite transfer of multigenic traits to crop varieties of diverse ecosystems. Over two decades of research has identified several DNA recombinases that carryout efficient cis and trans recombination between the recombination sites artificially introduced into the plant chromosome. The specificity and efficiency of recombinases make them extremely attractive for genome engineering. In plant biotechnology, recombinases have mostly been used for removing selectable marker genes and have rarely been extended to more complex applications. The reversibility of recombination, a property of the tyrosine family of recombinases, does not lend itself to gene stacking approaches that involve rounds of transformation for integrating genes into the engineered sites. However, recent developments in the field of recombinases have overcome these challenges and paved the way for gene stacking. Some of the key advancements include the application of unidirectional recombination systems, modification of recombination sites and transgene site modifications to allow repeated site-specific integrations into the selected site. Gene stacking is relevant to agriculturally important c...Continue Reading

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Mar 31, 2016·Plant Cell Reports·Teodoro Cardi, C Neal Stewart
Dec 19, 2015·The Plant Journal : for Cell and Molecular Biology·Holger Puchta
Jun 15, 2018·The Plant Journal : for Cell and Molecular Biology·Ray CollierRoger Thilmony
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Oct 1, 2021·Plant Cell Reports·Zhifen ZhangPeggy Ozias-Akins

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