Gene structure and enzymatic activity of mouse eosinophil-associated ribonuclease 2

Gene
A L McDevittKimberly D Dyer

Abstract

Mouse eosinophil-associated ribonuclease-2 (mEAR-2) is one of a cluster of genes identified in the genome of the mouse Mus musculus that are highly divergent orthologs of the primate ribonucleases, eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP). Northern analysis revealed expression of genes hybridizing to mEAR-2 in mouse lung, liver and spleen tissues. We obtained full-length cDNA by hybridization screening of mouse eosinophil and lung cDNA libraries and by rapid amplification of cDNA ends (RACE) from liver, spleen and lung RNA. Using these methods we have isolated the 195 base pair (bp) 3' untranslated region (UTR) that includes a typical polyadenylation signal preceding a poly A tail and the 5' UTR which includes 63-71 bp and three distinct transcriptional start sites. Using unidirectional PCR we isolated a 361-bp 5' promoter region and delineated the intronic / exonic boundaries which include a non-coding exon 1, a single intron, and a coding exon 2, a structure that is typical of genes of the RNase A superfamily. Consensus sites for PU.1 and EoTF, both active as intronic enhancer elements of the gene encoding EDN, are also present in the intron of the gene encoding mEAR-2. The catalytic activity ...Continue Reading

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Citations

Aug 21, 2003·Antiviral Research·Joanne M MoreauHelene F Rosenberg
Jul 6, 2013·Journal of Proteome Research·Hongde LiYulan Wang
Nov 22, 2002·American Journal of Respiratory Cell and Molecular Biology·Stephania A CormierJames J Lee
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jianzhi Zhang, Helene F Rosenberg
Mar 6, 2007·The Journal of Allergy and Clinical Immunology·Michio FujiwaraHiroshi Nakajima
Aug 7, 2009·European Journal of Immunology·Masanori MiyataAtsuhito Nakao
Mar 29, 2002·Molecular Biology and Evolution·Jianzhi Zhang, Helene F Rosenberg
Feb 26, 2015·The Journal of Biological Chemistry·Kelsey J YamadaHelene F Rosenberg

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