Gene structure and transcriptional regulation of dnaK and dnaJ genes from a psychrophilic bacterium, Colwellia maris

Extremophiles : Life Under Extreme Conditions
Seiji YamauchiHidenori Hayashi

Abstract

The dnaK and dnaJ genes, encoding heat shock proteins, were cloned from a psychrophilic bacterium, Colwellia maris. Significant homology was evident comparing DnaK and DnaJ of the psychrophilile with the counterparts of mesophilic and thermophilic bacteria. In the DnaJ protein, three conserved regions of the Hsp40 family were observed. A putative promoter similar to the sigma32 consensus sequence was found upstream of the dnaK gene. The G+C content in the 5'-untranslated region of the dnaK gene was much lower than that in the corresponding region of mesophilic bacteria. Northern-blot analysis and primer-extension analysis showed that both genes were transcribed separately as monocistronic mRNAs. Following several temperature upshifts from 10 to 26 degrees C, maximum induction of the dnaK and dnaJ mRNAs was detected at 20 degrees C, suggesting that this temperature induces the heat shock response in this bacterium. In addition, the level of the induction of the dnaJ gene was much lower than that of the dnaK gene. These findings together revealed several specific features of the heat shock response at a relatively low temperature in psychrophiles.

References

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Citations

Dec 20, 2005·Extremophiles : Life Under Extreme Conditions·Seiji YamauchiHidenori Hayashi
Sep 22, 2012·Extremophiles : Life Under Extreme Conditions·Seiji YamauchiHidenori Hayashi
Feb 5, 2010·Journal of Bioscience and Bioengineering·Morio IshikawaYukimichi Koizumi

Related Concepts

Bacterial Proteins
DNA, Bacterial
Impacts, Environmental
Heat shock proteins
Gene Expression Regulation, Bacterial
Determination, Sequence Homology
Homologous Sequences, Amino Acid
Oligonucleotide Primers

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