Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans

Molecular and Biochemical Parasitology
S KrauseEva Liebau

Abstract

Two highly similar genes encoding unique extracellular, glycosylated glutathione S-transferases (GSTs) of the human-pathogenic nematode, Onchocerca volvulus (Ov-GST1a and Ov-GST1b), have been isolated and characterised. The genes are approximately 3 kb in length and consist of seven exons interrupted by introns of approximately 100 bp in length, with the exception of intron II, which is approximately 1.6 kb in length. Interestingly, exon I and II encode a signal peptide and an N-terminal extension before sequence homology to other GSTs begins. The 5' flanking region was sequenced and analysed for transcription factor binding sites. Consistent with the lack of a TATA box, analysis of the mRNAs by primer extension showed multiple transcription start sites spread over a 60 bp region. To examine the activity and specificity of the Ov-GST1a gene promoter, we have exploited Caenorhabditis elegans as a heterologous transformation system. To analyse whether transgenic C. elegans are able to carry out processing and post-transcriptional modifications of the Ov-GST1a correctly, the protein was ectopically overexpressed in C. elegans. The parasite-derived Ov-GST1a gene product was correctly processed in transgenic C. elegans and posttrans...Continue Reading

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Citations

Jul 17, 2008·Comparative and Functional Genomics·William F Gregory, John Parkinson
Sep 19, 2003·International Journal for Parasitology·Jon P Boyle, Timothy P Yoshino
May 22, 2003·Free Radical Biology & Medicine·Britta LeiersKimberly Henkle-Dührsen
Nov 8, 2002·Parasitology International·Darren R Brooks, R Elwyn Isaac
Dec 28, 2004·International Journal for Parasitology·Eva U PetterssonJens G Mattsson
Sep 13, 2005·Journal of Molecular Biology·Tarig B HigaziThomas R Unnasch
Mar 24, 2011·Drug Metabolism Reviews·Jack U Flanagan, Mark L Smythe
Apr 30, 2009·International Journal for Parasitology·Victoria GillanEileen Devaney
Jun 12, 2009·Drug Metabolism Reviews·Viktor CvilinkLenka Skálová

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