PMID: 9161032Apr 1, 1997Paper

Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome

The Plant Journal : for Cell and Molecular Biology
E RisseeuwP J Hooykaas

Abstract

To develop a model system for studies of homologous recombination in plants, transgenic Nicotiana tabacum and Nicotiana plumbaginifolia lines were generated harbouring a single target T-DNA containing the negative selective codA gene encoding cytosine deaminase (CD) and the beta-glucuronidase (GUS) gene. Subsequently, the target lines were transformed with a replacement-type T-DNA vector in which the CD gene and the GUS promoter had been replaced with a kanamycin-resistance gene. For both Nicotiana species kanamycin-resistant lines were selected which had lost the CD gene and the GUS activity. One tobacco line was the result of a precise gene targeting event. However, most other lines were selected due to a chromosomal deletion of the target locus. The deletion frequency of the target locus varied between target lines, and could be present in up to 20% of the calli which were grown from leaf protoplasts. T-DNA transfer was not required for induction of the deletions, indicating that the target loci were unstable. A few lines were obtained in which the target locus had been deleted partially. Sequence analysis of the junctions revealed deletion of DNA sequences between microhomologies. We conclude that T-DNAs, which are stable d...Continue Reading

Citations

Mar 1, 1993·Plant Molecular Biology·C Lichtenstein, E Barrena
Jun 29, 2005·Molecular Genetics and Genomics : MGG·John G JeleskoWilhelm Gruissem
May 4, 2005·Transgenic Research·Olivier Cotsaftis, Emmanuel Guiderdoni
Apr 5, 2001·Trends in Plant Science·S Kumar, M Fladung
Oct 26, 2002·Trends in Plant Science·Animesh Ray, Marybeth Langer
Mar 6, 1999·Current Opinion in Plant Biology·D R Gallie
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Xiaoxia ZhaoL Alexander Lyznik
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Stanton B Gelvin
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Stanton B. Gelvin
Oct 22, 2008·BMC Genetics·Andrew BottleyRobert M D Koebner
Jan 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alan LloydGary N Drews
Aug 28, 2003·Journal of Plant Physiology·Holger Puchta
Dec 20, 2011·GM Crops·Amjad Masood HusainiBushra Aquil
May 30, 2021·Proceedings of the National Academy of Sciences of the United States of America·Oliver Xiaoou Dong, Pamela C Ronald

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.