Gene targeting and transgene stacking using intra genomic homologous recombination in plants

Plant Methods
Sandeep KumarMichelle Smith

Abstract

Modern agriculture has created a demand for plant biotechnology products that provide durable resistance to insect pests, tolerance of herbicide applications for weed control, and agronomic traits tailored for specific geographies. These transgenic trait products require a modular and sequential multigene stacking platform that is supported by precise genome engineering technology. Designed nucleases have emerged as potent tools for creating targeted DNA double strand breaks (DSBs). Exogenously supplied donor DNA can repair the targeted DSB by a process known as gene targeting (GT), resulting in a desired modification of the target genome. The potential of GT technology has not been fully realized for trait deployment in agriculture, mainly because of inefficient transformation and plant regeneration systems in a majority of crop plants and genotypes. This challenge of transgene stacking in plants could be overcome by Intra-Genomic Homologous Recombination (IGHR) that converts independently segregating unlinked donor and target transgenic loci into a genetically linked molecular stack. The method requires stable integration of the donor DNA into the plant genome followed by intra-genomic mobilization. IGHR complements conventio...Continue Reading

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Citations

Sep 25, 2016·Planta·Sandeep KumarJoseph F Petolino
Apr 8, 2017·Plant Biotechnology Reports·C C M van de WielM J M Smulders
Dec 22, 2016·Cellular and Molecular Life Sciences : CMLS·Claudia P Spampinato
Nov 10, 2017·Emerging Topics in Life Sciences·Sandeep KumarStephen Novak

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Methods Mentioned

BETA
transgenic

Software Mentioned

IGHR

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