Gene therapy for adult T cell leukemia using human immunodeficiency virus vector carrying the thymidine kinase gene of herpes simplex virus type 1

Human Gene Therapy
K ObaruK Takatsuki

Abstract

Adult T cell leukemia/lymphoma (ATL) is derived from CD4+ T cells and has a poor prognosis because of its resistance to chemotherapy. To evaluate the effectiveness of gene therapy for ATL, the effect of ganciclovir on ATL cell lines transfected with the thymidine kinase gene of herpes simplex virus type 1 (HSV-TK) was analyzed. To transfer the HSV-TK gene to ATL cells, a human immunodeficiency virus (HIV) vector that has specific infectivity to CD4+ cells was used. HSV-TK was inserted into the long terminal repeats of HIV-1 and driven by the SL3 promoter HXBSL3TK. HXBSL3TK was co-transfected with HXBCAT as a reporter into MT2 or HUT102 cells by DEAE-dextran. The cells were incubated with ganciclovir, and chloramphenicol acetyltransferase (CAT) activity was analyzed. The CAT activity of the MT2 cells and HUT102 cells transfected with HXBSL3TK decreased dose-dependently with ganciclovir. HXBSL3TK was also co-transfected into COS cells with an HIV-1 packaging vector that has gag, pol, and env driven by a cytomegalovirus promoter. The supernatant was transferred to MT2 cells or Raji cells and incubated with ganciclovir. Ninety percent of the MT2 cells transduced by HXBSL3TK and incubated with ganciclovir were killed, but Raji cells...Continue Reading

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A TanakaM Hatanaka
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E F TerwilligerW A Haseltine
Jul 1, 1988·Reviews of Infectious Diseases·J P SommadossiW C Buhles
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H HoshinoT Sugimura
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Aug 1, 1981·The Journal of Experimental Medicine·L E PosnerR C Gallo
Mar 1, 1995·The Journal of General Virology·J H RichardsonA M Lever
Jun 19, 1993·International Journal of Cancer. Journal International Du Cancer·M KannagiS Harada

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Citations

Feb 12, 1999·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·Y KitamuraK Yoshiike
Sep 17, 1998·Japanese Journal of Cancer Research : Gann·H KodairaK Ozawa
Dec 14, 2016·Nature Communications·Francisco Úbeda, Vincent A A Jansen

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