PMID: 9535452Apr 16, 1998Paper

Gene therapy in lung transplantation: effective gene transfer via the airways

The Journal of Thoracic and Cardiovascular Surgery
A JeppssonC G McGregor

Abstract

Gene therapy may provide a means of modifying factors that contribute to the development of pathologic processes in transplanted lungs. Experiments were designed to study the feasibility of adenovirus-mediated gene transfer by way of the airways to the transplanted lung. Orthotopic left lung transplantation (Lewis to Lewis rats) was performed on four groups of animals. 300 microl of adenovirus solution encoding for beta-galactosidase was infused into the left bronchus of donor rats at viral concentrations of 10(8) pfu/ml (n = 5), 10(9) pfu/ml (n = 6), and 10(10) pfu/ml (n = 6), and the lung was ventilated for 5 minutes. Controls (n = 6) received medium only. Seven days after transplantation, native and transduced, transplanted lungs were harvested. Sections of lung were fixed and stained with a solution of X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) and staining was evaluated for distribution by cell type and intensity. Beta-galactosidase expression was absent in the control group and in the native lungs. Two of five lungs in the 10(8) group expressed beta-galactosidase, but in a limited distribution and intensity. All six lungs in the 10(9) group and five of six lungs in the 10(10) group expressed beta-galactos...Continue Reading

References

May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y YangJ M Wilson
Apr 1, 1995·The Journal of Thoracic and Cardiovascular Surgery·A ArdehaliA J Lusis
Oct 1, 1993·Circulation·M D Schneider, B A French
Jan 1, 1993·The Journal of Clinical Investigation·A MastrangeliR G Crystal

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Citations

Sep 2, 1998·The Annals of Thoracic Surgery·A JeppssonC G McGregor
Apr 10, 1999·The Annals of Thoracic Surgery·A Haverich
Dec 28, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·S D CassiviS Keshavjee
Dec 9, 2000·Transplant International : Official Journal of the European Society for Organ Transplantation·A JeppssonC G McGregor
Mar 27, 2001·European Journal of Clinical Investigation·T Parpala-SpårmanK Tryggvason

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